LysoSensor™ Yellow/Blue DND-160 - Special Packaging
LysoSensor™ Yellow/Blue DND-160 - Special Packaging
Invitrogen™

LysoSensor™ Yellow/Blue DND-160 - Special Packaging

LysoSensor Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such asRead more
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Catalog NumberQuantity
L754520 x 50 μL
Catalog number L7545
Price (USD)
516.00
Each
Add to cart
Quantity:
20 x 50 μL
Price (USD)
516.00
Each
Add to cart
LysoSensor Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pKa of LysoSensor Yellow/Blue DND-160 is ∼4.2.

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For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 mM
Detection MethodFluorescence
Quantity20 x 50 μL
Shipping ConditionRoom Temperature
Sub Cellular LocalizationLysosomes
ColorYellow, Blue
Emission329/440
For Use With (Equipment)Fluorescence Microscope
Product LineLysoSensor
Product TypeDye
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Can I use a plate reader to measure the pH of acidic organelles, such as lysosomes, with LysoSensor ratiometric probes?

Using a plate reader for measuring the pH of acidic organelles, such as lysosomes, with LysoSensor ratiometric probes, provides weak pH measurements, as discrete regions of interest cannot be selected for analysis. Therefore, there is no way of excluding extracellular fluorescence and little to no spatial or morphological information can be obtained. Instead, a readout of whole-cell fluorescence is provided, resulting in the average intraorganellar pH of all labeled organelles for the analyzed cell population. The is also the risk that a signal may be dominated by a few extremely bright, but essentially irrelevant cells, such as dead cells, or dye-labeled debris particles.

For more information about measuring luminal pH in organelles, please see the reference linked below:

Live-cell Microscopy and Fluorescence-based Measurement of Luminal pH in Intracellular Organelles

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What reagents would you recommend for measuring the pH in acidic organelles, such as lysosomes?

For measuring the pH in acidic organelles, such as lysosomes, we recommend our LysoSensor ratio metric probes. Please see the links to the product page and descriptions below:

LysoSensor Yellow/Blue dextran, 10,000 MW, Anionic, Fixable (Cat. No. L22460):
LysoSensor Yellow/Blue dextran is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. This dextran conjugate is taken up by the cells through endocytosis. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pKa of LysoSensor Yellow/Blue dextran is ~3.9.

LysoSensor Yellow/Blue DND-160 - Special Packaging (Cat. No. L7545):
LysoSensor Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pKa of LysoSensor Yellow/Blue DND-160 is ~4.2.

LysoSensor Blue DND-167 - Special Packaging (Cat. No. L7533):
LysoSensor Blue DND-167 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomes more fluorescent in acidic environments. The pKa of LysoSensor Blue DND-167 is ~5.1.

LysoSensor Green DND-189 - Special Packaging (Cat. No. L7535):
LysoSensor Green DND-189 can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye becomes more fluorescent in acidic environments. The pKa of LysoSensor Green DND-189 is ~5.2.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Could you provide me a protocol or reference for measuring the pH in acidic organelles, such as lysosomes?

Please find a link to a reference for measuring luminal pH in intracellular organelles below:

Live-cell Microscopy and Fluorescence-based Measurement of Luminal pH in Intracellular Organelles

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between LysoSensor Yellow/Blue dextran, 10,000 MW, Anionic, Fixable (Cat. No. L22460) and LysoSensor Yellow/Blue DND-160 - Special Packaging (Cat. No. L7545)?

LysoSensor Yellow/Blue dextran, 10,000 MW, Anionic, Fixable (Cat. No. L22460) has an emission of 335/452, is fixable and allows loading of cells by endocytosis. The pKa of LysoSensor Yellow/Blue dextran is ~3.9, which is slightly lower than LysoSensor Yellow/Blue DND-160 - Special Packaging (Cat. No. L7545).

LysoSensor Yellow/Blue DND-160 - Special Packaging (Cat. No. L7545) has an emission of 329/440 and is unique as it shows both dual-excitation and dual-emission spectral peaks that are pH-dependent. Nevertheless, this LysoSensor only exhibits the pH-dependent dual-emission spectra in living cells. Lysosensor Yellow/Blue DND-160 has predominantly yellow fluorescence, and in less acidic organelles is has blue fluorescence. Dual-emission measurements may permit ratio imaging of the pH in acidic organelles such as lysosome.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the recommended incubation time for LysoTracker Green DND-26 (Cat. No. L7526) and LysoSensor Yellow/Blue DND-160 (Cat. No. L7545)?

LysoTracker Green DND-26 (Cat. No. L7526) and LysoSensor Yellow/Blue DND-160 (Cat. No. L7545) show faster uptake in cells and consequently a faster "alkalizing effect" on the lysosomes, so we recommend incubating the cells with these probes for 1-5 min at 37 degrees C. As the other LysoTracker and LysoSensor probes have a slower rate of uptake, we recommend a longer incubation time of 30 min to 2 hr.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.