Invitrogen™

Novex™ deutlich vorgefärbter Proteinstandard

Katalognummer	Menge
LC5800	2 x 250 μl

Katalognummer LC5800

Preis (EUR)

342,00

Menge:

2 x 250 μl

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

342,00

Der Novex Sharp vorgefärbte Proteinstandard ist für präzise, einfache und anwenderfreundliche Molekulargewichtsschätzung für Proteine verschiedenster Molekulargewichte bei SDS-PAGE und Western Blotting ausgelegt. Der Standard besteht aus 12 farbigen Banden im Bereich von 3,5 bis 260 kDa und wird in einem gebrauchsfertigen Format für die direkte Beladung auf Gele geliefert. Vor der Verwendung muss kein Probenpuffer erwärmt, reduziert oder hinzugefügt werden.

Alle anderen Proteinstandards und -leitern anzeigen und vergleichen ›

Anwendungen
• Überwachen der Proteinmigration während der SDS-Polyacrylamid-Gelelektrophorese
• Überwachen der Proteinübertragung auf Membranen nach Western Blotting
• Größenbestimmung von Proteinen auf SDS-PAGE-Gelen und Western Blots

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

NachweisverfahrenKolorimetrisch

GelkompatibilitätBolt™ Bis-Tris Plus-Gele, Novex™ Tricin-Gele, Novex™ Tris-Glycin-Gele, NuPAGE™ Bis-Tris-Gele

Molekulargewicht260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3,5 kDa

Menge2 x 250 μl

Sofort einsatzbereitJa

VersandbedingungZugelassen für den Versand auf Nass- oder Trockeneis

Number of Markers12

ProduktlinieNovex

ProdukttypProteinleiter

Größenbereich3,5 bis 260 kDa

Stain Type3 Farben: Rosa, Gelb, Blau

System TypeWestern Blotting, SDS-PAGE

Unit SizeEach

Inhalt und Lagerung

Vorgefärbte Novex™ Sharp Proteinstandards, geliefert als 2x 250 μL gebrauchsfertige Standardmischung (insgesamt 50 Anwendungen mit je 10 μL). Vorgefärbter Novex™ Sharp Standardladepuffer aus 65 mM Tris pH-Wert 6,5, 30 % Glycerin, 2 % SDS und 2,5 mM ETDA.

Lagerung bei -20 °C.

Häufig gestellte Fragen (FAQ)

Why can I not see the 3.5 kDa band of the Invitrogen Sharp Pre-Stained Standard on my NuPAGE gels?

The 3.5 kDa band is visible only on NuPAGE gels with 1X MES SDS running buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the different molecular weight estimations for the Invitrogen Sharp Pre-Stained standard on the different Invitrogen gel types?

Invitrogen Sharp Pre-Stained Standard molecular weight estimations are the same in NuPAGE Bis-Tris, NuPAGE Tris-Acetate, Invitrogen Tris-Glycine, and Tricine Gels. The appearance/migration of the protein bands may differ depending upon the gel type and running conditions

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the recommended protocols for preparing Invitrogen protein standards to load on a gel?

Invitrogen protein standards do not require any additional preparation. Thaw protein standards at room temperature, vortex to mix well, and load.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used one of your pre-stained protein standards for a western transfer and I noticed that the intensity of the band faded from the membrane during the transfer process. Why is this?

The fading is most likely due to detergent in the western blocking/washing solutions that can remove some of the proteins from the membrane. The dye itself will not wash off of the proteins because it is covalently bound. We have found that smaller pore size membranes retain the proteins better during blocking and wash procedures, and hence recommend use of 0.2 µm instead of 0.45 µm membranes for best resolution and protein retention. After transfer, it is a good idea to circle the pre-stained bands with a pencil on the membrane, so band positions can be identified after blocking and processing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your protein standards for a western transfer and noticed that some of the lower-molecular weight protein bands passed through the membrane. How can I resolve this issue?

- Decrease voltage, current or length of transfer time
- Make sure that the methanol concentration in the transfer buffer is proper; use a methanol concentration of 10-20% methanol removes the SDS from SDS-protein complexes and improves the binding of protein to the membrane.
- Make sure that the SDS concentration (if added) in the transfer buffer is proper, don't use more than 0.02-0.04% SDS. Using too much SDS can prevent binding of proteins to the membrane.
- Check the pore size of the membrane and the size of the target protein. Proteins smaller than 10 kDa will easily pass through a 0.45 µm pore size membrane. If proteins smaller than 10 kDa are of interest, it would be better to use a 0.2 µm pore size membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Novex™ deutlich vorgefärbter Proteinstandard FAQs