Cytokine 29-Plex Monkey Panel
Cytokine 29-Plex Monkey Panel
Invitrogen™

Cytokine 29-Plex Monkey Panel

The Monkey Cytokine Magnetic 29-Plex Panel for the Luminex™ platform is specifically designed for quantifying monkey cytokines, chemokines and growth深入閱讀
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產品號碼Quantity
LPC0005M100 tests
產品號碼 LPC0005M
價格 (HKD)
-
Quantity:
100 tests

The Monkey Cytokine Magnetic 29-Plex Panel for the Luminex™ platform is specifically designed for quantifying monkey cytokines, chemokines and growth factors in serum, plasma, and tissue culture supernatant samples. By measuring 29 analytes simultaneously, the Luminex™ assay panel helps provide more data from each sample, saving both money and time compared to more traditional systems (such as ELISA) used in research. This assay may be run alone or in combination with other select Luminex™ singleplex kits. The panel employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The panel is suitable for use with the Luminex™ 100™/200™, FLEXMAP 3D™, and MAGPIX™ systems.

Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
High quality—fully qualified antibodies permit excellent specificity and sensitivity
Fast and easy protocols—perform your multiplex assay and analyze your data typically in less than one day

The Monkey Cytokine Magnetic 29-Plex Panel for the Luminex™ platform provides reagents for the accurate, reproducible, and sensitive quantitation of monkey proteins including:

CytoKines: IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-15, IL-17, G-CSF, GM-CSF, IFN-γ, IP-10, TNF-α

Chemokines: Eotaxin, IL-8, MCP-1, MDC, MIF, MIG, MIP-1α, MIP-1β, I-TAC, RANTES

Growth factors: EGF, FGF-basic, HGF, VEGF

Luminex™ xMAP™ Technology—Effective and Proven Analytical Tool
The Monkey Cytokine Magnetic 29-Plex Panel is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. Upon completion of the sandwich immunoassay, magnetic beads must be measured using a Luminex™ detection system (MAGPIX™, 100™/200™, or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

Magnetic Beads Let You Do More with Your Time
Magnetic bead-based assays use Luminex™ MagPlex magnetic microsphere. All other assay components are the same as the equivalent Luminex™ polystyrene bead-based assays providing identical quality and consistency performance. MagPlex technology takes advantage of magnetic properties to simplify assay wash steps and maximize uniformity of results. Magnetic bead-based assays also enable automation, decrease hands-on time, and increase throughput and precision.

The Monkey Cytokine Magnetic 29-Plex Panel for the Luminex™ platform is specifically designed for quantifying monkey cytokines, chemokines and growth factors in serum, plasma, and tissue culture supernatant samples. By measuring 29 analytes simultaneously, the Luminex™ assay panel helps provide more data from each sample, saving both money and time compared to more traditional systems (such as ELISA) used in research. This assay may

The Monkey Cytokine Magnetic 29-Plex Panel for the Luminex™ platform is specifically designed for quantifying monkey cytokines, chemokines and growth factors in serum, plasma, and tissue culture supernatant samples. By measuring 29 analytes simultaneously, the Luminex™ assay panel helps provide more data from each sample, saving both money and time compared to more traditional systems (such as ELISA) used in research. This assay may be run alone or in combination with other select Luminex™ singleplex kits. The panel employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The panel is suitable for use with the Luminex™ 100™/200™, FLEXMAP 3D™, and MAGPIX™ systems.

Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
High quality—fully qualified antibodies permit excellent specificity and sensitivity
Fast and easy protocols—perform your multiplex assay and analyze your data typically in less than one day

The Monkey Cytokine Magnetic 29-Plex Panel for the Luminex™ platform provides reagents for the accurate, reproducible, and sensitive quantitation of monkey proteins including:

CytoKines: IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-15, IL-17, G-CSF, GM-CSF, IFN-γ, IP-10, TNF-α
Chemokines: Eotaxin, IL-8, MCP-1, MDC, MIF, MIG, MIP-1α, MIP-1β, I-TAC, RANTES
Growth factors: EGF, FGF-basic, HGF, VEGF

Luminex™ xMAP™ Technology—Effective and Proven Analytical Tool
The Monkey Cytokine Magnetic 29-Plex Panel is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. Upon completion of the sandwich immunoassay, magnetic beads must be measured using a Luminex™ detection system (MAGPIX™, 100™/200™, or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

Magnetic Beads Let You Do More with Your Time
Magnetic bead-based assays use Luminex™ MagPlex magnetic microsphere. All other assay components are the same as the equivalent Luminex™ polystyrene bead-based assays providing identical quality and consistency performance. MagPlex technology takes advantage of magnetic properties to simplify assay wash steps and maximize uniformity of results. Magnetic bead-based assays also enable automation, decrease hands-on time, and increase throughput and precision. Easy to use protocols allow you to get your results in as little as 3.5 hours. In addition, these assays are compatible with both vacuum and magnetic wash stations and work well with the MAGPIX™ system as well as other Luminex™ xMAP™ platforms. (Figure 1).

More Validation for Confidence in Your Results
The Monkey Cytokine Magnetic 29-Plex Panel has been validated using the same rigorous criteria that distinguishes Luminex™ assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. When benchmarked against the competition, our multiplex products consistently deliver excellent results (Figure 2). Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay (Figure 3; Documents section below).

Learn more about all Invitrogen™ assays for the Luminex™ platform.

For Research Use Only. Not for use in diagnostic procedures.
規格
Assay RangeSee product documentation
Assay SensitivitySee Certificate of Analysis
Bead TypeSee product documentation
ConjugateR-PE
Detection MethodFluorescence
For Use With (Equipment)Luminex™ Instruments
FormatPreconfigured Panel
Product LineNovex
ProteinCytokine
Protein SubtypeCytokines & Receptors
Sample TypeSerum, Plasma, Cell Culture Supernatants
Shipping ConditionWet Ice
CombinabilityCombinable
Product TypeMultiplex Panel
Quantity100 tests
Research AreaImmunology, Cytokines
SpeciesNon-Human Primate
Unit SizeEach
內容物與存放
Contains premixed antibody coated capture beads, standard, premixed detector antibody, diluents, SAV concentrate, buffers, wash solution, flat bottom plate, complete protocol and lot-specific technical data sheet. Store at 2°C to 8°C.

常見問答集 (常見問題)

During my ProcartaPlex assay data analysis, I am getting a warning message that there is high bead aggregation. What should I do?

Here are possible causes and solutions for this issue:

- Check the protocol settings (make sure you select the correct DD settings).
- Check the level of sheath fluid and empty the waste.
- Before acquiring the plate, run calibration and verification beads on the Luminex instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

During my ProcartaPlex assay data analysis, the beads fall below or to the lower left of their bead region on the bead map. Why is this?

Here are possible causes and solutions for this issue:

This usually indicates that the beads have been photobleached. This problem can also be caused by exposing the beads to organic solvents. Unfortunately, the assay will have to be repeated because the beads cannot be restored. The beads must be protected from light and organic solvents.
Alternatively, the instrument may be off in its measurements or you may have a calibration issue. Call the manufacturer for a service appointment.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

During my ProcartaPlex assay data analysis, beads do not appear in the region gated. What happened?

This indicates that an incorrect buffer was used for the final step. The Wash Solution provided in the kit must be used for washing the beads and the Reading Buffer should be used for resuspending the beads before loading them into the Luminex instrument. The osmolarity of the solution will impact the size of the bead, and any change in the bead size will alter detection by the instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The bead counts for all of my ProcartaPlex assay wells are erratic. What went wrong?

Here are some suggestions:

- Before acquiring the plate, run calibration and verification beads on the Luminex instrument.
- Review the instrument settings and make sure they are appropriate for the assay being run (adjustment of needle height, make sure you select the correct bead gates and the correct DD settings).
- Shake the plate before acquisition on the instrument to resuspend the beads.
- Vortex the beads for 30 sec before adding them into the plate.
- Washing: Do not forget to keep the plate for about 2 mins on the Hand-Held Magnetic Plate Washer before emptying the plate.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The bead count for my ProcartaPlex assay standard curve is correct and regular. The bead count for my samples is erratic and I get a warning message saying "The acquisition had at least one region that did not reach the maximum count". What should I do?

This pattern is indicative of a sample matrix effect. Here are some suggestions:

- Confirm that the sample has been clarified and is free of debris and free of lipids (5-10 min centrifugation recommended).
- Confirm that there is at least a 1:1 ratio of sample to assay diluent for serum, plasma samples. For cell lysates or tissue homogenates, confirm that the sample has been diluted appropriately in assay buffer to reduce the concentration of detergent in the lysis buffer to ⋜0.01%. For other sample types, further sample optimization may be required.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

引用資料與參考文獻 (6)

引用資料與參考文獻
Abstract
Intrabronchial infection of rhesus macaques with simian varicella virus results in a robust immune response in the lungs.
Authors:Haberthur K, Meyer C, Arnold N, Engelmann F, Jeske DR, Messaoudi I,
Journal:
PubMed ID:25142604
'Varicella-zoster virus (VZV) is the etiological agent of varicella (chickenpox) and herpes zoster (shingles). Primary VZV infection is believed to occur via the inhalation of virus either in respiratory droplets or from shedding varicella lesions or by direct contact with infectious vesicular fluid. However, the ensuing immune response in the ... More
Selective blockade of CD28-mediated T cell costimulation protects rhesus monkeys against acute fatal experimental autoimmune encephalomyelitis.
Authors:Haanstra KG, Dijkman K, Bashir N, Bauer J, Mary C, Poirier N, Baker P, Crossan CL, Scobie L, 't Hart BA, Vanhove B,
Journal:
PubMed ID:25589073
Costimulatory and coinhibitory receptor-ligand pairs on T cells and APC control the immune response. We have investigated whether selective blockade of CD28-CD80/86 costimulatory interactions, which preserves the coinhibitory CTLA4-CD80/86 interactions and the function of regulatory T (Treg) cells, abrogates the induction of experimental autoimmune encephalomyelitis (EAE) in rhesus monkeys. EAE ... More
Sex hormones selectively impact the endocervical mucosal microenvironment: implications for HIV transmission.
Authors:Goode D, Aravantinou M, Jarl S, Truong R, Derby N, Guerra-Perez N, Kenney J, Blanchard J, Gettie A, Robbiani M, Martinelli E,
Journal:
PubMed ID:24830732
Several studies suggest that progesterone and estrogens may affect HIV transmission in different, possibly opposing ways. Nonetheless, a direct comparison of their effects on the mucosal immune system has never been done. We hypothesize that sex hormones might impact the availability of cells and immune factors important in early stages ... More
Toll-Like Receptor 7/8 (TLR7/8) and TLR9 Agonists Cooperate To Enhance HIV-1 Envelope Antibody Responses in Rhesus Macaques.
Authors:Moody MA, Santra S, Vandergrift NA, Sutherland LL, Gurley TC, Drinker MS, Allen AA, Xia SM, Meyerhoff RR, Parks R, Lloyd KE, Easterhoff D, Alam SM, Liao HX, Ward BM, Ferrari G, Montefiori DC, Tomaras GD, Seder RA, Letvin NL, Haynes BF,
Journal:
PubMed ID:24390332
The development of a vaccine that can induce high titers of functional antibodies against HIV-1 remains a high priority. We have developed an adjuvant based on an oil-in-water emulsion that incorporates Toll-like receptor (TLR) ligands to test whether triggering multiple pathogen-associated molecular pattern receptors could enhance immunogenicity. Compared to single ... More
Intranasal infection and contact transmission of Zika virus in guinea pigs.
Authors:Deng YQ, Zhang NN, Li XF, Wang YQ, Tian M, Qiu YF, Fan JW, Hao JN, Huang XY, Dong HL, Fan H, Wang YG, Zhang FC, Tong YG, Xu Z, Qin CF
Journal:Nat Commun
PubMed ID:29162827
'Zika virus (ZIKV) is primarily transmitted to humans through mosquito bites or sexual contact. The excretion and persistence of contagious ZIKV in various body fluids have been well documented in ZIKV patients; however, the risk of direct contact exposure remains unclear. Here, we show that guinea pigs are susceptible to ... More