Thermo Scientific™

Maxima™ H Minus cDNA Synthesis Master Mix

Catalog number: M1661
Thermo Scientific™

Maxima™ H Minus cDNA Synthesis Master Mix

Catalog number: M1661
Catalog Number
M1661
Unit Size
50 reactions
Price (USD)
Price: 364.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
200 reactions
Price (USD)
Price: 1,142.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
M166150 reactions
Price: 364.00
Your Price:
***
M1662200 reactions
Price: 1,142.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Features include:
• One-tube master mix helps reduce pipetting steps and enhanced consistency in RT-qPCR results
• Increased RT efficiency across a wide range of input RNA amounts and gene targets
• High thermostability to allow RT reactions at 50 to 65°C temperature range

The master mix contains Maxima H Minus Reverse Transcriptase (RT) and RiboLock RNase Inhibitor. Maxima H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution. The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, and lack of RNase H activity. The recombinant RiboLock RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, and C at temperatures up to 55°C. The master mix also contains reaction buffer, dNTPs, oligo (dT)18, and random hexamer primers. A separate tube of No-RT control mix is also provided.

Maxima H Minus cDNA Synthesis Master Mix is capable of reproducible cDNA synthesis at elevated temperatures (50–65°C). The synthesis reaction is typically complete in 15–30 minutes.

Additional information about reaction components
• The No RT Control mix contains all the components in the Maxima H Minus cDNA Synthesis Master Mix except RT. The presence of a PCR product in the No RT Control reaction indicates that the reaction is contaminated with DNA. To further enhance genomic DNA elimination efficiency, template RNA incubation with dsDNase (Cat. No.EN0771) is strongly recommended.
• Nuclease-free water is provided for reaction set-up and dilution of sample DNA. The absence of exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.

Related products
Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase
Maxima H Minus First Strand cDNA Synthesis Kit
Maxima H Minus Reverse Transcriptase
dsDNase

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Real-Time PCR
Concentration
5X
Final Product Type
cDNA (First-Strand)
Format
Tube
No. of Reactions
50 Reactions
PCR Method
RT-PCR
Reaction Format
Master Mix
Reagent Type
Reverse Transcription
Sample Type
RNA
Size (Final Product)
Up to 20 kb
Starting Material
RNA
Technique
Reverse Transcription
Volume
200 μL
Kit Type
H Minus cDNA Synthesis Master Mix Kit
Optimal Reaction Temperature
50°C inculbation temperature for RT reactions
Product Line
Maxima™
Product Type
cDNA Synthesis Master Mix
Reverse Transcriptase
Maxima H Minus
Shipping Condition
Dry Ice
Sufficient For
500 Reactions

Contents & Storage

• 1 x 200 μL Maxima H Minus cDNA Synthesis Master Mix
• 1 x 200 μL Maxima H Minus cDNA Synthesis Master Mix No RT Control
• 1 x 1.25 mL nuclease-free water

Store at -5 to -30°C.

Figures