Kit de ensayo MitoProbe™ TMRM para citometría de flujo

Citas y referencias (4)

Thermo Scientific™

Kit de ensayo MitoProbe™ TMRM para citometría de flujo

El kit de ensayo TMRM de MitoProbe contiene tetrametilrodamina metiléster (TMRM) para la detección del estado potencial de la membranaMás información

Número de catálogo	Cantidad
M20036	100 ensayos

Número de catálogo M20036

Precio (MXN)

Cantidad:

100 ensayos

El kit de ensayo TMRM de MitoProbe contiene tetrametilrodamina metiléster (TMRM) para la detección del estado potencial de la membrana mitocondrial y CCCP para la inducción de la despolarización de la membrana mitocondrial, si se desea.

• Estudio del potencial de la membrana mitocondrial en células vivas
• Protocolo rápido y fácil

El estado de salud mitocondrial se puede determinar mediante la detección de cambios en el potencial de la membrana mitocondrial. En una célula sana con mitocondria activa, la TMRM se aísla fácilmente, emitiendo una señal fluorescente roja anaranjada. Cuando se induce la apoptosis o se aplica un tratamiento como CCCP, la membrana mitocondrial se despolariza y la señal de TMRM disminuye.

Especificaciones

Método de detecciónFluorescente

FormatoVial(es)

Cantidad100 ensayos

Condiciones de envíoHielo húmedo

Emission561

Para utilizar con (aplicación)Ensayo de TMRM

Para utilizar con (equipo)Citómetro de flujo

Tipo de productoKit de ensayo de citómetros de flujo

Unit SizeEach

Contenido y almacenamiento

1 vial de TMRM (100 pruebas)
1 vial de 50 mM de CCCP
Almacenar entre -5 ° y -30 °C.

Preguntas frecuentes

In the MitoProbe TMRM Assay Kit for Flow Cytometry, should CCCP be present when adding the TMRM?

Yes. The CCCP should be first added to the cell sample, incubated for 5 mins, and then the TMRM should be added and incubated for another 30 mins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How does the MitoProbe TMRM Assay Kit for Flow Cytometry (Cat. No.M20036) differ from the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is provided as a 100 µM solution of TMRM in DMSO (5 vials, 100 µL in each vial). The MitoProbe TMRM Assay Kit for Flow Cytometry provides the TMRM as a 20 µM stock solution in DMSO (1 vial, 100 µL) and a vial of CCCP. Both kits have the stock solutions as a 1000X concentration, but final working concentrations required for imaging applications are 5-fold higher than what is needed for flow cytometry analysis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (4)

Citations & References

Abstract

Mitochondrial Delivery of an Anticancer Drug Via Systemic Administration Using a Mitochondrial Delivery System That Inhibits the Growth of Drug-Resistant Cancer Engrafted on Mice.

Authors:Yamada Y, Munechika R, Satrialdi Kubota F, Sato Y, Sakurai Y, Harashima H

Journal:J Pharm Sci

PubMed ID:32376272

'Mitochondrial delivery of an anticancer drug targeting cancer cells would eventually result in cell death. To achieve this, a drug delivery system targeting mitochondria is needed. We recently developed a MITO-Porter, a liposome that delivers its cargo to mitochondria. We reported that such a MITO-Porter could deliver doxorubicin (DOX), an ... More

Zidovudine-Mediated Autophagy Inhibition Enhances Mitochondrial Toxicity in Muscle Cells.

Authors:Lin H, Stankov MV, Hegermann J, Budida R, Panayotova-Dimitrova D, Schmidt RE, Behrens GMN,

Journal:Antimicrob Agents Chemother

PubMed ID:30373793

Nucleoside reverse transcriptase inhibitors (NRTI), such as zidovudine (AZT), are constituents of HIV-1 therapy and are used for the prevention of mother-to-child transmission. Prolonged thymidine analogue exposure has been associated with mitochondrial toxicities to heart, liver, and skeletal muscle. We hypothesized that the thymidine analogue AZT might interfere with autophagy ... More

Lectin-induced oxidative stress in human platelets.

Authors:Signorello MG, Ravera S, Leoncini G

Journal:Redox Biol

PubMed ID:32063518

Previously we have shown that wheat germ agglutinin (WGA) and, with minor potency, Phaseolus vulgaris agglutinin (PHA), but not lens culinarian agglutinin (LCA), induce platelet aggregation, through the PLC?2 activation by the concerted action of src/syk and PI3K/BTK pathways. In this study, we have investigated platelet oxidative stress induced by ... More

Rapid Death of Follicular B Cells and Burkitt Lymphoma Cells Effectuated by Xbp1s.

Authors:Chen YT, Kung JT

Journal:J Immunol

PubMed ID:32376649

BCR-mediated tonic signaling is an indispensable requirement for the survival of follicular B (FOB) cells and Burkitt lymphoma (BL) cells. FOB cells of the I-A