The MitoProbe TMRM Assay Kit contains tetramethylrhodamine methyl ester (TMRM) for the detection of mitochondrial membrane potential state and CCCP for the induction of mitochondrial membrane depolarization, if desired.
• Study mitochondrial membrane potential in live cells • Quick, easy protocol
Determining the state of mitochondrial health can be achieved by detecting changes in the mitochondrial membrane potential. In a healthy cell with active mitochondria, TMRM is readily sequestered, thereby emitting a red-orange fluorescent signal. When apoptosis is induced or a treatment like CCCP is applied, the mitochondrial membrane is depolarized and the TMRM signal diminishes.
For Use With (Equipment)
Contents & Storage
1 vial TMRM (100 tests)
1 vial 50 mM CCCP
Store at -5°C to -30°C.
MitoProbe TMRM detects changes in mitochondrial membrane potential
Fluorescence spectra of MitoProbe TMRM Assay Kit for Flow Cytometry
Detection of mitochondrial potential changes in apoptotic cells