Human Large Vessel Endothelial Cell Basal Medium (formerly “Medium 200”) is a sterile, liquid tissue culture medium intended for use as one component in a complete culture environment for normal human large vessel endothelial cells. When supplemented with low serum growth supplement (LSGS), this medium supports the plating and proliferation of endothelial cells at culture densities between 2.5 x 10³ cells/cm² and 1 x 10⁵ cells/cm². Additional applications may include primary isolation of endothelial cells. This medium is also available without phenol red (Cat. No. M200PRF500).

Human Large Vessel Endothelial Cell Basal Medium is a basal culture medium containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. This medium does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. It is HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO²/95% air. To support the plating and proliferation of human large vessel endothelial cells, this medium must be supplemented with LSGS (Cat. No. S-003-10; kit = Cat. No. S-003-K).