Gibco™

Medium 254

Green features
Catalog number: M254500
Gibco™

Medium 254

Green features
Catalog number: M254500
Catalog Number
M254500
also known as M-254-500
Unit Size
500 mL
Price (USD)
87.25
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
M254500
also known as M-254-500
500 mL
87.25
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Medium 254 is a sterile, liquid tissue culture medium prepared with 200 µM calcium chloride for the culture of normal human epidermal melanocytes. This medium is intended for use as one component in a complete culture environment. When supplemented with HMGS or HMGS-2, Medium 254 will support the plating and proliferation of melanocytes at varying culture densities from 5 x 103 cells/cm2 to 1 x 105 cells/cm2. Additional applications for use may include primary isolation of melanocytes from skin.

Medium 254 is a basal medium containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. This medium does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. This medium is HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2/95% air. To support plating and long-term proliferation of normal human melanocytes, Medium 254 must be supplemented with Human Melanocyte Growth Supplement (HMGS) or PMA-Free Human Melanocyte Growth Supplement-2 (HMGS-2). Each of these supplements contains all of the growth factors, hormones, and tissue extracts necessary for growth of melanocytes in Medium 254.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Medium 254
Sterility
Sterile-filtered
Inorganic Salts
Calcium
Shipping Condition
Room Temperature
Product Line
Cascade Biologics™
Form
Liquid
Phenol Red Indicator
Phenol Red
Green Features
Sustainable packaging
Cell Type
Melanocyte Cell
With Additives
Phenol Red
Culture Type
Mammalian Cell Culture
Species
Human
Sterilization Method
Sterile Filtered
HEPES Buffer
HEPES

Contents & Storage

Store in refrigerator (2–8°C) and protect from light.

Media Formulations

Figures