MitoProbe™ DiOC2(3) Assay Kit - For Flow Cytometry - 100 Assays

Catalog number: M34150

Molecular Probes™  Related applications: Cell Structure | Cell Viability, Proliferation & Function | Flow Cytometry

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Description

The MitoProbe™ DiOC2(3) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiOC2(3), and a mitochondrial membrane-potential disrupter, CCCP, for the study of mitochondrial membrane potential. DiOC2(3) penetrates the cytosol of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, producing bright, red fluorescence. DiOC2(3) staining intensity decreases with decreased mitochondrial membrane potential, accompanied by a shift from red to green fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: DiOC2(3)
Sub-Cellular Localization: Mitochondria
Product Size: 1 kit
Detection Method: Fluorescent
Number of Reactions: 100
For Use With (Equipment): Flow Cytometer
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 482⁄497
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial of DiOC2(3) (625 µL, 10 µM in DMSO), and 1 vial of CCCP: (125 µL, 50 mM DMSO).

Store at 2–6°C, protected from light. Do not freeze.

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