Invitrogen™

MitoProbe™ JC-1 Assay Kit

Catalog number: M34152
Invitrogen™

MitoProbe™ JC-1 Assay Kit

Catalog number: M34152
Catalog Number
M34152
Unit Size
1 kit
Price (USD)
536.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
M341521 kit
536.00
Your Price:
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The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry.

View a selection guide for all apoptosis assays for flow cytometry.

Selective to mitochondrial changes
The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. JC-1 dye exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (∼529 nm) to red (∼590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates.

Unlike other mitochondrial stains that are affected by plasma membrane potential, the ratio of green to red fluorescence of JC-1 depends only on the membrane potential and not other factors such as mitochondrial size, shape, and density, which may influence single-component fluorescence signals. Use of fluorescence ratio detection allows researchers to make comparative measurements of membrane potential and determine the percentage of mitochondria within a population that respond to an applied stimulus.

Indicator of early apoptosis
A distinctive feature of the early stages of programmed cell death is the disruption of active mitochondria. This mitochondrial disruption includes changes in the membrane potential and alterations to the oxidation-reduction potential of the mitochondria. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting equilibration of ions leads in turn to the decoupling of the respiratory chain and the release of cytochrome c into the cytosol.

Simple, streamlined protocol
One of the biggest challenges to working with dual-emitting dyes like JC-1 is compensation. The MitoProbe™ JC-1 Assay Kit provides a mitochondrial membrane-potential disrupter, CCCP, in order to produce compensation controls leading to a correctly compensated green-to-red fluorescence ratio. The stain protocol is simple and straightforward: incubate the cells with JC-1 dye and then analyze the fluorescent signal in the FITC and PE channels of of a 488 nm laser.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission
514⁄529, 590
Flow Cytometer Laser Lines
488
For Use With (Application)
Flow Cytometry
For Use With (Equipment)
Flow Cytometer
Label or Dye
JC-1
No. of Reactions
100
Product Line
MitoProbe™
Shipping Condition
Room Temperature
Sub Cellular Localization
Mitochondria
Detection Method
Fluorescent
Format
Tube

Contents & Storage

Contains 5 vials of JC-1 (5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide) (30 µg/vial of powdered dye), 1 vial of DMSO (1.5 mL), 1 vial of CCCP (125 µL , 50 mM in DMSO), and 1 bottle of 10X phosphate-buffered saline (25 mL).

Stored at 2–8°C. Protect from light.

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