Invitrogen™

MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging

Catalog number: M36008
Invitrogen™

MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging

Catalog number: M36008
MitoSOX Green and MitoSOX Red superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.
 
Catalog Number
M36008
Unit Size
10 x 50 µg
Indicator
MitoSox Red
Color
Red
Excitation/Emission
396/610 nm
Quantity
10 Vials
Price (USD)
Price: 373.00
Your Price:
Availability
-
Full specifications
Quantity
ColorRed
Detection MethodFluorescence
Excitation/Emission396/610 nm
IndicatorMitoSox Red
Product LineMitoSOX™
FormatVial(s)
Quantity10 Vials
Unit Size10 x 50 µg
Catalog Number
Unit Size
9 µg
Indicator
MitoSox Green
Color
Green
Excitation/Emission
488/510 nm
Quantity
1 Vial
Price (USD)
Price: 153.00
Your Price:
Availability
-
Full specifications
Quantity
ColorGreen
Detection MethodFluorescence
Excitation/Emission488/510 nm
IndicatorMitoSox Green
Product LineMitoSOX™
Quantity1 Vial
Unit Size9 µg
Catalog Number
Unit Size
5 x 9 µg
Indicator
MitoSox Green
Color
Green
Excitation/Emission
488/510 nm
Quantity
5 Vials
Price (USD)
Price: 399.00
Your Price:
Availability
-
Full specifications
Quantity
ColorGreen
Detection MethodFluorescence
Excitation/Emission488/510 nm
IndicatorMitoSox Green
Product LineMitoSOX™
Quantity5 Vials
Unit Size5 x 9 µg
Catalog Number
Unit Size
50 µg
Indicator
MitoSox Red
Color
Red
Excitation/Emission
396/610 nm
Quantity
1 Vial
Price (USD)
Price: 153.00
Your Price:
Availability
-
Full specifications
Quantity
ColorRed
Detection MethodFluorescence
Excitation/Emission396/610 nm
IndicatorMitoSox Red
Product LineMitoSOX™
Quantity1 Vial
Unit Size50 µg
Catalog Number
Unit Size
2 vials
Indicator
MitoSox Green & MitoSox Red
Color
Green, Red
Excitation/Emission
488/510 nm (green), 396/610 nm (red)
Quantity
2 Vials (1 Green, 1 Red)
Price (USD)
Price: 250.00
Your Price:
Availability
-
Full specifications
Quantity
ColorGreen, Red
Detection MethodFluorescence
Excitation/Emission488/510 nm (green), 396/610 nm (red)
IndicatorMitoSox Green & MitoSox Red
Product LineMitoSOX™
Quantity2 Vials (1 Green, 1 Red)
Unit Size2 vials
Showing 5 of 5
Catalog NumberSpecificationsUnit SizeIndicatorColorExcitation/EmissionQuantityPrice (USD)AvailabilityQuantity
M36008Full specifications
10 x 50 µgMitoSox RedRed396/610 nm10 Vials
Price: 373.00
Your Price:
-
ColorRed
Detection MethodFluorescence
Excitation/Emission396/610 nm
IndicatorMitoSox Red
Product LineMitoSOX™
FormatVial(s)
Quantity10 Vials
Unit Size10 x 50 µg
M36005Full specifications
9 µgMitoSox GreenGreen488/510 nm1 Vial
Price: 153.00
Your Price:
-
ColorGreen
Detection MethodFluorescence
Excitation/Emission488/510 nm
IndicatorMitoSox Green
Product LineMitoSOX™
Quantity1 Vial
Unit Size9 µg
M36006Full specifications
5 x 9 µgMitoSox GreenGreen488/510 nm5 Vials
Price: 399.00
Your Price:
-
ColorGreen
Detection MethodFluorescence
Excitation/Emission488/510 nm
IndicatorMitoSox Green
Product LineMitoSOX™
Quantity5 Vials
Unit Size5 x 9 µg
M36007Full specifications
50 µgMitoSox RedRed396/610 nm1 Vial
Price: 153.00
Your Price:
-
ColorRed
Detection MethodFluorescence
Excitation/Emission396/610 nm
IndicatorMitoSox Red
Product LineMitoSOX™
Quantity1 Vial
Unit Size50 µg
M36009Full specifications
2 vialsMitoSox Green & MitoSox RedGreen, Red488/510 nm (green), 396/610 nm (red)2 Vials (1 Green, 1 Red)
Price: 250.00
Your Price:
-
ColorGreen, Red
Detection MethodFluorescence
Excitation/Emission488/510 nm (green), 396/610 nm (red)
IndicatorMitoSox Green & MitoSox Red
Product LineMitoSOX™
Quantity2 Vials (1 Green, 1 Red)
Unit Size2 vials
Showing 5 of 5
Features of these indicators include:
• Readily oxidized by superoxide but not by other ROS- or RNS-generating systems
• Use for live cell imaging
• Rapidly and selectively targeted to the mitochondria
• MitoSOX Green indicator: absorption/emission maxima of ∼488/510 nm (traditional FITC/GFP filter set)
• MitoSox Red indicator: absorption/emission maxima of ∼396/610 nm* (custom filter set recommended)

*Robinson et al. (1) reported that if MitoSOX Red dye is excited at 396 nm instead of 510 nm, it can be a more selective marker for mitochondrial superoxide.

MitoSOX Green indicator is offered as one vial or a pack of five vials. MitoSOX Red indicator is offered as one vial or a pack of ten vials. A variety pack of one vial each of MitoSOX Red and MitSOX Green indicators is also available.

Quick, easy detection of mitochondrial superoxide in live cells
The production of superoxide by mitochondria can be visualized in fluorescence microscopy using MitoSOX superoxide indicators. They permeate live cells where they selectively target mitochondria. They are rapidly oxidized by superoxide but not by other reactive oxygen species (ROS) and reactive nitrogen species (RNS). The oxidized product is highly fluorescent.

MitoSOX indicators may be used to distinguish artifacts of isolated mitochondrial preparations from direct measurements of superoxide generated in the mitochondria of live cells. They may also provide a valuable tool in the research of agents that modulate oxidative stress in various pathologies. In addition, these indicators have been used in metabolic flux assays using high-content instruments (2). MitoSOX indicators have also been used to detect mitochondrial superoxide using flow cytometery (3).

References
1: Robinson, Kristine M., et al. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proceedings of the National Academy of Sciences 103.41 (2006): 15038-15043.
2: Little, Andrew Charles, et al. High-content fluorescence imaging with the metabolic flux assay reveals insights into mitochondrial properties and functions. Communications biology 3.1 (2020): 1-10.
3: Kauffman, Megan E. et al. MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS. Reactive oxygen species (Apex, N.C.) vol. 2,5 (2016): 361-370.

For Research Use Only. Not for use in diagnostic procedures.