MitoSOX™ Red reagent is a novel fluorogenic dye specifically targeted to mitochondria in live cells. Oxidation of MitoSOX™ Red reagent by superoxide produces red fluorescence.
• Readily oxidized by superoxide but not by other ROS- or RNS-generating systems • Absorption/emission maxima: ~510/580 nm • Use for live cell imaging • Rapidly and selectively targeted to the mitochondria Quick, Easy Detection of Mitochondrial Superoxide in Live Cells The production of superoxide by mitochondria can be visualized in fluorescence microscopy using the MitoSOX™ Red reagent. MitoSOX™ Red reagent permeates live cells where it selectively targets mitochondria. It is rapidly oxidized by superoxide but not by other reactive oxygen species (ROS) and reactive nitrogen species (RNS). The oxidized product is highly fluorescent upon binding to nucleic acid.
MitoSOX™ Red reagent may be used to distinguish artifacts of isolated mitochondrial preparations from direct measurements of superoxide generated in the mitochondria of live cells. It may also provide a valuable tool in the discovery of agents that modulate oxidative stress in various pathologies.
For Research Use Only. Not for use in diagnostic procedures.