BenchPro® 2100 Plasmid Processing Station

Catalog number: MC1001

Invitrogen™  Related applications: Automated Nucleic Acid Purification | DNA Extraction

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Description

The BenchPro® 2100 Plasmid Purification System is an innovative, automated instrument that provides a completely walk-away solution for high quality purification of your large-scale plasmid DNA.
• Innovative, straight-from-culture automation
• Superior yield and purity
• Minimize hands-on work and human errors

Innovative, Straight-From-Culture Automation
Going straight from culture to purified plasmid, the BenchPro® 2100 simplifies the standard, 22-step process of manual plasmid purification down a few easy set-up steps that can be performed typically in less than 5 minutes. Assemble the Reagent Tray and Cell Liner on the Waste Tray, and add your 125-ml E. coli culture to the Cell Liner. Then load the BenchPro® 2100 Plasmid Purification Card into one of the instrument slots, and run the purification protocol.

Unparalleled Yield and Purity
The BenchPro® 2100 Plasmid Purification Card at the core of the instrument is a disposable, fluidics card that contains a novel anion-exchange membrane that provides the same high-quality plasmid DNA as our manual PureLink® HiPure kits provide. Each plasmid preparation can be trusted for use in many applications – mammalian cell transfection, automated and manual sequencing, PCR amplification, cloning, transformation, and in vitro transcription. The BenchPro® 2100 system is able to reproducibly purify a range of plasmid sizes, from 2.7 to 20 kb (Figure 1). Plasmid yields from the BenchPro® 2100 are unmatched by those achieved by alternative methods (Figure 2).

Minimizes Variation and User Error
The BenchPro® 2100 Plasmid Purification System is designed to provide consistency and reliability by using prefilled and sealed reagents. All required buffers in appropriate volumes are supplied in a sealed Reagent Tray, and a disposable Cell Liner (for the addition of culture) with lid is included. The BenchPro® 2100 Plasmid Purification System accurately performs all steps of the purification process to help deliver consistent yield (Figure 3), eliminating the tedious and repetitive liquid-handling steps associated with the manual process of plasmid purification. During the automated purification protocol, pre-defined amounts of each reagent are sequentially pumped into the card, and incubation steps performed according to the protocol. Using a series of capture and purification membranes, E. coli cells are harvested and lysed, and the lysate clarified. By minimizing the number of hands-on steps, the BenchPro® 2100 Plasmid Purification System enhances experimental reproducibility and data consistency run after run.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Proprietary Card
Capacity: 1 or 2 preps per run
Quantity: 1 each
Dimensions: 13.0 in. (w) x 20.8 in. (d) x 11.7 in. (h)
Product Size: 1 each
Input Voltage: 100-240 VAC
Plan Duration: 12 Months
Reaction Time: 90 min
Shipping Condition: Room Temperature
Compatible Products: BenchPro® 2100 Piercing Device, BenchPro® 2100 Plasmid Purification Card and Reagent Tray Kit, BenchPro® Waste Tray
Sample Loading Volume: 100-125 ml bacterial culture
For Use With (Equipment): BenchPro® 2100 Plasmid Purification System
High Throughput Compatibility: Automated Protocols

Contents & storage

BenchPro® 2100 Plasmid Processing Station
AC⁄DC Switching Power Adapter
2 Waste Trays
Piercing device
Quick-disconnect house air fitting including 12 feet of tubing
Quick Reference Card (QRC)
Instruction Manual

Documents

Manuals & protocols