Gibco™

EpiLife™ Medium, with 60 µM calcium

Green features
Catalog number: MEPI500CA
Gibco™

EpiLife™ Medium, with 60 µM calcium

Green features
Catalog number: MEPI500CA
Catalog Number
MEPI500CA
also known as M-EPI-500-CA
Unit Size
500 mL
Price (USD)
105.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
MEPI500CA
also known as M-EPI-500-CA
500 mL
105.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
EpiLife is a sterile, liquid medium prepared with 60 µM calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal medium requires the addition of an appropriate growth supplement prior to use. EpiLife Medium greatly extends the in vitro lifespan of human epidermal keratinocytes compared to other serum-free media systems. This basal media is animal origin-free and contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. It does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. EpiLife Medium is HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2 and 95% air.

To support plating and long term proliferation of human keratinocytes, EpiLife Medium must be supplemented with selected hormones and growth factors. We offer a choice of growth supplements for culturing human keratinocytes. Please refer to the EpiLife Medium user guide to determine the best supplement choice for your purposes. The supplements contain all of the growth factors, hormones, and extracts necessary for growth of human keratinocytes in EpiLife Medium.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
EpiLife Medium
Sterility
Sterile-filtered
Inorganic Salts
Calcium
Shipping Condition
Room Temperature
Product Line
Cascade Biologics™, EpiLife™
Form
Liquid
Phenol Red Indicator
Phenol Red
Green Features
Sustainable packaging
Cell Type
Epithelial Cell (Corneal), Keratinocyte Cell
With Additives
Phenol Red
Culture Type
Mammalian Cell Culture
Species
Human
Sterilization Method
Sterile Filtered
Classification
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
HEPES Buffer
HEPES
Media Type
Basal Media
Serum Level
Serum-Free

Contents & Storage

Store in refrigerator (2–8°C) and protect from light.

Media Formulations

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