Thermo Scientific™

Lab Vision™ Retinoblastoma (Rb) Ab-1, Mouse Monoclonal Antibody

Related applications:

Anatomical Pathology

Provide accurate, reproducible results with the Thermo Scientific™ Retinoblastoma (Rb) Ab-1, Mouse Monoclonal Antibody.
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Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Host Species: Mouse

Clone: 1F8; same as Rb1

Isotype: IgG1

Species Reactivity: Human. Does not react with mouse and rat. Others not known.

Epitope: aa 703-722

Immunogen: Recombinant human Rb protein

Molecular Weight: 105kDa

Positive Control: Ls174T, HT29, HUVEC cells; Normal colon, Breast CA and colon CA

Cellular Localization: Nuclear Ab-1 specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It reacts with hyperphosphorylated and un (under) phosphorylated form of Rb protein. It shows no cross reaction with p107 and p130.

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation (Native and denatured)
  • Flow Cytometry

Note:

USA: RUO; Int'l: RUO

General References:

1. Bartek J, et. al. Oncogene 7:101-108, 1992.

2. Grand R et. al. Oncogene. 4:1291-1298, 1989.