Thermo Scientific™

Lab Vision™ Vinculin Ab-1, Mouse Monoclonal Antibody

Related applications:

Anatomical Pathology

Provide accurate, reproducible results with the Thermo Scientific™ Vinculin Ab-1, Mouse Monoclonal Antibody.
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Vinculin is an attachment protein involved in the indirect binding of intracellular actin filaments to extracellular fibronectin. Vinculin binds to actin capping protein and α actinin, which in turn binds to actin. The binding of fibronectin is also indirect where vinculin binds to talin, which in turn binds to the intracellular domain of the fibronectin receptor which itself is the ligand for fibronectin. Vinculin is widely distributed in tissue and is expressed where smooth muscle actin and fibroblasts attach to the extracellular matrix.

Host Species: Mouse

Clone: VLN01

Isotype: IgG

Species Reactivity: Human. Others not known.

Epitope: Not determined

Immunogen: Semi-purified vinculin

Molecular Weight: 130kDa (vinculin) and 150kDa (meta-vinculin)

Positive Control: HeLa cells, skin

Cellular Localization: Cell membrane and cytoplasmic Ab-1 is useful for studies of vinculin in platelet activation.

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation (Denatured verified)

Note:

USA: RUO; Int'l: RUO

General References:

1. Molecular Biology of the Cell. 2nd Edition. Garland Publishing Inc. Page 636.

2. Asijee G M, et al. European Journal of Biochemistry. 189: 131-136 (1990).