Thermo Scientific™

Lab Vision™ Chk2 Ab-1, Mouse Monoclonal Antibody

 Related applications:
Anatomical Pathology

Ensure accurate, reproducible results in western blotting, immunofluorescence and immunoprecipitation experiments with Thermo Scientific™ Chk2 Ab-1, Mouse Monoclonal Antibody.

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Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phophorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Host Species: Mouse

Clone: 2CHK01; same as DCS-293

Isotype: IgG1/κ

Species Reactivity: Human. Others not known.

Epitope: Not determined

Immunogen: Recombinant Chk2 protein

Molecular Weight: ∽70 kDa

Positive Control: HeLa cells

Cellular Localization: Nuclear

Recommended for:

  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation (Native verified)


USA: RUO; Int'l: RUO

General References:

1. Wu X, et. al. (2001) J Biol Chem, 276:2971-2974.


Manuals & protocols