Ensure accurate, reproducible results in western blotting, immunofluorescence and immunoprecipitation experiments with Thermo Scientific™ Chk2 Ab-1, Mouse Monoclonal Antibody.
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phophorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
Host Species: Mouse
Clone: 2CHK01; same as DCS-293
Species Reactivity: Human. Others not known.
Epitope: Not determined
Immunogen: Recombinant Chk2 protein
Molecular Weight: âˆ½70 kDa
Positive Control: HeLa cells
Cellular Localization: Nuclear
USA: RUO; Int'l: RUO
1. Wu X, et. al. (2001) J Biol Chem, 276:2971-2974.