Thermo Scientific™

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-2, Mouse Monoclonal Antibody

Related applications:

Anatomical Pathology

Ensure accurate, reproducible results in immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-2, Mouse Monoclonal Antibody.
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EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, βcellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Host Species: Mouse

Clone: 225

Isotype: IgG1

Species Reactivity: Human, Chimpanzee and Monkey. Does not react with mouse and rat. Others not known.

Epitope: Extracellular domain

Immunogen: Purified EGFR from A431 cells

Molecular Weight: 170 kDa (wild type) and 145 kDa (vIII variant)

Positive Control: A431 cells

Cellular Localization: Membrane

Recommended for:

  • Immunoprecipitation (Native only)
  • Blocks EGF/TGFα-Induced Activation of EGFR
  • Arrests Tumor Growth in vivo
  • Inhibits Binding of Ab-1 (clone 528) to EGFR

Note:

USA: RUO; Int'l: RUO

General References:

1. Kawamoto T, et. al. Prc Natl Acad Sci, USA, 1983, 80:1337-41.

2. Sato JD, et. al. Mol Biol Med, 1983, 1:511-29.

3. Wikstrand CJ, et. al. Cancer Res, 1995, 55:3140-8.