Thermo Scientific™

Lab Vision™ Estrogen Receptor Ab-10, Mouse Monoclonal Antibody

 Related applications:
Anatomical Pathology
Ensure accurate, reproducible results in western blotting, immunoprecipitation, gel supershift and chromatin precipitation experiments with Thermo Scientific™ Estrogen Receptor Ab-10, Mouse Monoclonal Antibody.

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The ER gene consists of more than 140kb of genomic DNA divided into 8 exons and is translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Host Species: Mouse

Clone: TE111.5D11

Isotype: IgG1

Species Reactivity: Human, Cow, Pig, Dog, Mouse, Rat, Sheep, Rabbit, Hamster and Chicken. Others not known.

Epitope: aa 302-553

Immunogen: 35 kDa, C-terminal fragment (aa 302-595) of human ER expressed in E. coli

Molecular Weight: 67 kDa

Positive Control: T47D cells

Cellular Localization: Nuclear

Recommended for:

  • Western Blotting
  • Immunoprecipitation (Native and denatured)
  • Gel Supershift
  • Blocks Binding of Estrogen to ER
  • Chromatin Precipitation


USA: RUO; Int'l: RUO

General References:

1. Abbondanza C et. al. Steroids, 1993, 58:4-12.


Manuals & protocols