Thermo Scientific™

Lab Vision™ MMP-2 (72kDa Collagenase IV) Ab-1, Mouse Monoclonal Antibody

Related applications:

Anatomical Pathology

Ensure accurate, reproducible results in experiments with the Thermo Scientific™ MMP-2 (72kDa Collagenase IV) Ab-1, Mouse Monoclonal Antibody.
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MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPs and TIMPs play a significant role in regulating angiogenesis. MMP-2; also known as 72kDa collagenase IV or gelatinase A is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids into an active form.of 62kDa.

Host Species: Mouse

Clone: CA-4001 or CA719E3C

Isotype: IgG1

Species Reactivity: Human, Mouse and Rat. Others not known.

Epitope: N-terminal (APSPIIKFPGD-VAPKTDK)

Immunogen: N-terminal peptide APSPIIKFPGD-VAPKTDK of procollagenase IV

Molecular Weight: 72kDa (proform)

Positive Control: Conditioned, serum-free medium from (TPA-treated) HFL-1 cells

Cellular Localization: Cytoplasmic

Recommended for:

  • Western Blotting
  • Immunoprecipitation (Not suitable)
  • Inhibition of MMP-2 Activity

Note:

USA: RUO; Int'l: RUO

General References:

1. Autio-Harmainen H, et al. Lab Invest, 1993, 69:312-321.

2. Schnaper H W, et al. J Cell Physiol, 1993, 156:235-246.

3. Fridman R, et al. J Biol Chem, 1992, 267:15398-15405.

4. Autio-Harmainen H, et al. Lab Invest, 1992, 67:191-200.

5. Soini Y, et. al. J Histochem Cytochem, 1994, 42:945-51.

6. Margulies IM, et. al. Cancer Epidemiology, Biomarkers and Prevention, 1992, 1(6):467-74.

7. Nikkari S T, et al. Am J PAthol, 1996, 149:1427-1433.