Thermo Scientific™

Lab Vision™ Insulin Receptor (α -Subunit) Ab-1, Mouse Monoclonal Antibody

Related applications:

Anatomical Pathology

Ensure accurate, reproducible results in experiments with the Thermo Scientific™ Insulin Receptor (α-Subunit) Ab-1, Mouse Monoclonal Antibody.
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The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a Β-α-α-Β configuration. The Β-subunit (95kDa) possesses a single transmembrane domain, whereas the α-subunit (135kDa) is completely extracellular.

Host Species: Mouse

Clone: 83-14

Isotype: IgG2a/κ

Species Reactivity: Human. Weakly with Cow, Pig and Sheep. Does not react with rabbit, mouse and rat. Others not known.

Epitope: aa 469-592 (exon 7/8)

Immunogen: IM-9 lymphocytes followed by purified insulin receptor

Molecular Weight: 135kDa

Positive Control: IM-9 lymphocyte cells, Placenta

Cellular Localization: Membrane Ab-1 inhibits insulin binding and mimics insulin action. It is excellent for immunoprecipitation of alpha-subunit of IR.

Recommended for:

  • Immunoprecipitation
  • ELISA
  • Insulin-like Agonist
  • Inhibits (∽80%) Insulin Binding (10nM)
  • Tyrosine Kinase Assay (Ab-mediated capture on microplates)

Note:

USA: RUO; Int'l: RUO

General References:

1. Taylor R, et. al. Biochem J, 1987, 242(1):123-9.

2. Soos MA, et. al. Biochem J, 1986, 235(1):199-208.

3. Prigent SA, et. al. J Biol Chem, 1990, 265:9970-7.

4. Soos MA, et. al. Biochem J, 1989, 263(2):553-63.

5. Soos MA, et. al. Proc Natl Acad Sci USA, 1989, 86(14):5217-21.

6. Zhang B, et. al. Proc Natl Acad Sci USA, 1991, 88:9858-62.