Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Invitrogen™

Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes

The Neon NxT Electroporation System 100-μL Kit is designed specifically for use with the Neon NxT Electroporation System.
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Número de catálogoN.º de reacciones
N1009696 × 2 reacciones
N1002525 × 2 reacciones
Número de catálogo N10096
Precio (USD)
3.701,70
Each
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N.º de reacciones:
96 × 2 reacciones
Precio (USD)
3.701,70
Each
Añadir al carro de la compra

The Neon NxT Electroporation System 100-μL Kit with 1-Channel Tubes is designed specifically for use with the Neon NxT Electroporation System (Cat. Nos. NEON1S, NEON1SK, NEON18S, NEON18SK). Use this kit for transfection volumes of 100 μL, containing 5 x 105–2 x 106 adherent cells or 1 x 106–5 x 106 suspension cells. Cells that have been transfected using the included 100-μL Neon NxT tips are then ready to be washed and plated into a multi-well culture dish.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Para utilizar con (aplicación)Transfección
Para utilizar con (equipo)Neon™ NxT Electroporation System
N.º de reacciones96 × 2 reacciones
Tipo de productoPipette Tip
Cantidad96 x 2 reactions
Volumen de la muestra (métrico)100 μL
Tipo de célulaStem Cells, Primary Cells, Hard-to-Transfect Cells
FormatoElectroporation Pipette Tip
Transfection TechniqueElectroporación
Unit SizeEach
Contenido y almacenamiento
• 4 x 10 mL Resuspension R Buffer; store at 4°C
• 4 x 10 mL Resuspension T Buffer; store at 4°C
• 4 x 10 mL Resuspension Genome Editing Buffer; store at 4°C
• 2 x 100 mL Electrolytic E100 Buffer; store at 4°C
• 96 reaction tips
• 32 electroporation 1-channel tubes

Preguntas frecuentes

Should I warm Neon NxT buffers before use?

We recommend warming the buffers to room temperature before use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I aliquot and store Neon NxT buffers?

We recommend storing Neon NxT buffers in the original containers at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What temperature should Neon NxT buffers be stored at?

Neon NxT buffers should be stored at 4 degrees C. Temperature fluctuations and storage at room temperature have been shown to increase the chances for precipitate formation. Therefore, the desired amount of buffer for an experiment should be removed from the buffer bottle and the bottle should be immediately returned to 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What state should the cells be in for best results with the Neon Nxt Electoporation System?

For best results with the Neon Nxt Electoporation System, the cells should be healthy (not stressed) and actively dividing so that the nuclear membrane integrity is already weakened for payload needing to enter the nucleus. Consider “starving” the cells of serum or other essential nutrients.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I'm experiencing arcing issues with the Neon Nxt Electoporation System. What could be causing this?

Arcing could be caused by high voltage or pulse length settings, high salt or contaminants in the DNA sample, incorrect cell density, and air bubbles formed during mixing of cell samples. Mix samples gently to avoid air bubble formation and pipette samples in a slow, smooth motion to avoid air uptake.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.