In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy.
AuthorsJung JC, Mehta AD, Aksay E, Stepnoski R, Schnitzer MJ
JournalJ Neurophysiol
PubMed ID15128753
'One of the major limitations in the current set of techniques available to neuroscientists is a dearth of methods for imaging individual cells deep within the brains of live animals. To overcome this limitation, we developed two forms of minimally invasive fluorescence microendoscopy and tested their abilities to image cells ... More
Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF.
AuthorsAlsina B, Vu T, Cohen-Cory S
JournalNat Neurosci
PubMed ID11593233
'Dynamic developmental changes in axon arbor morphology may directly reflect the formation, stabilization and elimination of synapses. We used dual-color imaging to study, in the live, developing animal, the relationship between axon arborization and synapse formation at the single cell level, and to examine the participation of brain-derived neurotrophic factor ... More
Macrophages kill T9 glioma tumor cells bearing the membrane isoform of macrophage colony stimulating factor through a phagocytosis-dependent pathway.
AuthorsJadus MR, Williams CC, Avina MD, Ly M, Kim S, Liu Y, Narasaki R, Lowell CA, Wepsic HT
JournalJ Immunol
PubMed ID9551992
Rat T9 glioma cells transfected with the gene for the membrane isoform of macrophage-CSF (mM-CSF) but not for the secreted isoform of M-CSF were directly killed by bone marrow-derived macrophages. Macrophage-mediated cytolysis of the mM-CSF-transfected clone was blocked by using chemical inhibitors of phagocytosis such as iodoacetate, 2-deoxyglucose, gadolinium chloride, ... More
Partition of membrane probes in a gel/fluid two-component lipid system: a fluorescence resonance energy transfer study.
AuthorsLoura LM, Fedorov A, Prieto M
JournalBiochim Biophys Acta
PubMed ID10930513
A non-ideal lipid binary mixture (dilauroylphosphatidylcholine/distearoylphosphatidylcholine), which exhibits gel/fluid phase coexistence for wide temperature and composition ranges, was studied using photophysical techniques, namely fluorescence anisotropy, lifetime and resonance energy transfer (FRET) measurements. The FRET donor, N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-dilauroylphosphatidylethanol amine, and a short-tailed FRET acceptor, 1,1'-didodecil-3,3,3',3'-tetramethylindocarbocyanine (DiIC12(3)), were shown to prefer the fluid ... More
A discrete stage of baculovirus GP64-mediated membrane fusion.
AuthorsKingsley DH, Behbahani A, Rashtian A, Blissard GW, Zimmerberg J
JournalMol Biol Cell
PubMed ID10588652
Viral fusion protein trimers can play a critical role in limiting lipids in membrane fusion. Because the trimeric oligomer of many viral fusion proteins is often stabilized by hydrophobic 4-3 heptad repeats, higher-order oligomers might be stabilized by similar sequences. There is a hydrophobic 4-3 heptad repeat contiguous to a ... More
Fever-range hyperthermia enhances L-selectin-dependent adhesion of lymphocytes to vascular endothelium.
AuthorsWang WC, Goldman LM, Schleider DM, Appenheimer MM, Subjeck JR, Repasky EA, Evans SS
JournalJ Immunol
PubMed ID9551935
The L-selectin leukocyte adhesion molecule plays an important role in controlling leukocyte extravasation in peripheral lymph nodes and at sites of tissue injury or infection. Although febrile responses during infection and inflammation are associated with enhanced immune activity, the contribution of fever-range temperatures to controlling lymphocyte recruitment to tissues has ... More
In Vivo Study of Dynamics and Stability of Dendritic Spines on Olfactory Bulb Interneurons in Xenopus laevis Tadpoles.
Authors
JournalPLoS One
PubMed ID26485435
SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.
Authors
JournalFront Synaptic Neurosci
PubMed ID31736736
Neurogenesis of retinal ganglion cells is not essential to visual functional recovery after optic nerve injury in adult zebrafish.
Authors
JournalPLoS One
PubMed ID23437359
Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo.
Authors
JournalData Brief
PubMed ID33748355
Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo.