Evaluation of glucose transport and its regulation by insulin in human monocytes using flow cytometry.
AuthorsDimitriadis G, Maratou E, Boutati E, Psarra K, Papasteriades C, Raptis SA
JournalCytometry A
PubMed ID15688355
'BACKGROUND: We investigated the effects of insulin on glucose transport in human monocytes using flow cytometry, a method with several advantages over previously used techniques. We hypothesized that monocytes could be used as tools to study insulin action at the cellular level and facilitate the investigation of mechanisms that lead ... More
Glutamate triggers rapid glucose transport stimulation in astrocytes as evidenced by real-time confocal microscopy.
AuthorsLoaiza A, Porras OH, Barros LF
JournalJ Neurosci
PubMed ID12917367
'Glutamate stimulates glycolysis in astrocytes, a phenomenon that couples astrocytic metabolism with neuronal activity. However, it is not known whether glutamate also affects glucose transporter-1 (GLUT1), the transporter responsible for glucose entry into astrocytes. To address this question, two different real-time single-cell hexose uptake assays were applied to cultured hippocampal ... More
Net sugar transport is a multistep process. Evidence for cytosolic sugar binding sites in erythrocytes.
AuthorsCloherty EK, Sultzman LA, Zottola RJ, Carruthers A
JournalBiochemistry
PubMed ID7492539
'Human erythrocyte net sugar transport is hypothesized to be rate-limited by reduced cytosolic diffusion of sugars and/or by reversible sugar association with intracellular macromolecules [Naftalin, R.J., Smith, P.M., & Roselaar, S.E. (1985) Biochim. Biophys. Acta 820, 235-249]. The present study examines these hypotheses. Protein-mediated 3-O-methylglucose uptake at 4 degrees C ... More
Expression of GLUT-2 cDNA in human B lymphocytes: analysis of glucose transport using flow cytometry.
AuthorsRauchman MI, Wasserman JC, Cohen DM, Perkins DL, Hebert SC, Milford E, Gullans SR
JournalBiochim Biophys Acta
PubMed ID1420258
'The molecular characterization of transport proteins is often limited by transient functional expression or the need for a simple method to select functional cDNA clones. We used a mammalian expression system to obtain long-term expression of GLUT-2, an isoform of glucose permease. Rat GLUT-2 cDNA was ligated into an EBV ... More
Flow cytometric analysis of glucose transport by rat brain cells.
AuthorsAller CB, Ehmann S, Gilman-Sachs A, Snyder AK
JournalCytometry
PubMed ID9041115
'The fluorescent, non-metabolizable glucose analog 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (NBDG) was used to measure rates of hexose transport by dissociated brain cells from developing and adult rats. Flow cytometric analysis of glucose uptake and expression of glucose transporters was performed by mapping on size by granularity, which discriminated between neurons and astrocytes in ... More
Regional differences in glucose transport in the mouse hippocampus.
AuthorsShimada M, Kawamoto S, Hirose Y, Nakanishi M, Watanabe H, Watanabe M
JournalHistochem J
PubMed ID8206790
'In order to observe glucose transport into the brain, 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (NBDG), a non-metabolizable and fluorescent glucose analogue, was injected intravenously into mice. After ascertaining that this glucose analogue is non-metabolizable in the brain, the NBDG contents in the blood and brain were measured quantitatively by spectrofluorimetry at 0, 0.5, 2, ... More
Membrane electroporation--fast molecular exchange by electroosmosis.
AuthorsDimitrov DS, Sowers AE
JournalBiochim Biophys Acta
PubMed ID1690573
'Human and rabbit erythrocyte ghosts loaded with FITC-dextran (mol. mass = 10 kDa) and NBD-glucosamine (mol. mass = 342 Da) in buffers of different ionic strength and composition were subjected to electric pulses (intensity 0.7 kV/mm and decay half-time 1 ms) at 7-10 degrees C and 20-24 degrees C. The ... More
AuthorsRouach N, Koulakoff A, Abudara V, Willecke K, Giaume C,
JournalScience
PubMed ID19056987
Astrocytes provide metabolic substrates to neurons in an activity-dependent manner. However, the molecular mechanisms involved in this function, as well as its role in synaptic transmission, remain unclear. Here, we show that the gap-junction subunit proteins connexin 43 and 30 allow intercellular trafficking of glucose and its metabolites through astroglial ... More
Electropore diameters, lifetimes, numbers, and locations in individual erythrocyte ghosts.
AuthorsSowers AE, Lieber MR
JournalFEBS Lett
PubMed ID3743774
Low light level video microscopy was used to study the diameter, lifetime, number, and location characteristics of electric field-induced pores (electropores) in erythrocyte ghosts. The diameter of electropores was probed by following the efflux of soluble fluorescent-tagged molecules out of the resealed ghost cytoplasmic compartments. After reaching a peak radius ... More
Kinetic validation of 6-NBDG as a probe for the glucose transporter GLUT1 in astrocytes.
AuthorsBarros LF, Bittner CX, Loaiza A, Ruminot I, Larenas V, Moldenhauer H, Oyarzún C, Alvarez M,
JournalJ Neurochem
PubMed ID19393014
In recent years, the use of fluorescent glucose analogs has allowed the study of rapid transport modulation in heterogeneous cell cultures and complex tissues. However, the kinetic behavior of these tracers is not conventional. For instance, the fluorescent glucose analog 6-NBDG permeates the cell 50-100 times slower than glucose but ... More
Glucose Directly Links to Lipid Metabolism through High Affinity Interaction with Peroxisome Proliferator-activated Receptor {alpha}.
AuthorsHostetler HA, Huang H, Kier AB, Schroeder F,
JournalJ Biol Chem
PubMed ID18055466
The pathophysiology of diabetes is characterized not only by elevated glucose but also elevated long chain fatty acid levels. We show for the first time that the peroxisome proliferator-activated receptor-alpha (PPARalpha) binds glucose and glucose metabolites with high affinity, resulting in significantly altered PPARalpha secondary structure. Glucose decreased PPARalpha interaction ... More
Asymmetric transport of a fluorescent glucose analogue by human erythrocytes.
AuthorsSpeizer L, Haugland R, Kutchai H
JournalBiochim Biophys Acta
PubMed ID4039191
A fluorescent glucose analogue, 6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-aminoglucose (NBDG), was synthesized and its interactions with the hexose transport system of the human red blood cell were investigated. NBDG entry is inhibited by increasing concentrations of D-glucose (Ki = 2 mM). However, NBDG exit is unaffected by D-glucose in red blood cells. Cytochalasin B ... More
A long-lived fusogenic state is induced in erythrocyte ghosts by electric pulses.
AuthorsSowers AE
JournalJ Cell Biol
PubMed ID3958050
Treatment of erythrocyte ghosts in random positions in a suspension with membrane fusion-inducing direct current electric field pulses causes the membranes to become fusogenic. Significant fusion yields are observed if the membranes are dielectrophoretically aligned into membrane-membrane contact with a weak alternating electric field as much as 5 min after ... More
Single-cell profiling reveals the origin of phenotypic variability in adipogenesis.
Authors
JournalPLoS One
PubMed ID19357775
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).
Authors
JournalEur J Immunol
PubMed ID31633216
Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.
Authors
JournalImmunity
PubMed ID29166588
Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming.
Authors
JournalNat Commun
PubMed ID32681081
Nephrotoxicity and Kidney Transport Assessment on 3D Perfused Proximal Tubules.
Authors
JournalAAPS J
PubMed ID30109442
Monocarboxylate transporter 1 deficiency impacts CD8+ T lymphocytes proliferation and recruitment to adipose tissue during obesity.
Authors
JournaliScience
PubMed ID35707720
Lactate Modulates Cellular Metabolism Through Histone Lactylation-Mediated Gene Expression in Non-Small Cell Lung Cancer.
Authors
JournalFront Oncol
PubMed ID34150616
Guidelines for the use of flow cytometry and cell sorting in immunological studies.
Authors
JournalEur J Immunol
PubMed ID29023707
D-2-hydroxyglutarate interferes with HIF-1α stability skewing T-cell metabolism towards oxidative phosphorylation and impairing Th17 polarization.
Authors
JournalOncoimmunology
PubMed ID29900057
Glutamine sensitivity analysis identifies the xCT antiporter as a common triple-negative breast tumor therapeutic target.