Thermo Scientific™

ELISA Blocker Blocking Buffer

Catalog number:  N502

 Related applications:

Protein Assays and Analysis

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ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.

Features OF THE ELISA Blocking Buffer:

Size: 1L (sufficient for thirty 96-well plates)
Application: Use as a blocking buffer in ELISA. The buffer is ready to use. No dilution is required.
Instructions for use in an ELISA: Following the incubation with a coating antibody, aspirate the coating antibody from the plate and add 300 µL of ELISA Blocker Blocking Buffer to each well. Cover the plate with a plate sealer and incubate for a minimum of 1 hour at room temperature (22-25°C). Aspirate blocking buffer and proceed with the ELISA procedure or dry the plate for future use.
Plate Drying: Plates should be dried uncovered, at room temperature, for a minimum of 1 hour. For optimal drying, plates should not be stacked and humidity should be at 30-40%. If plates are stacked, or humidity is high, the drying time should be increased. Once drying is complete, seal each plate in a foil pouch with a desiccant. When sealed with a desiccant, plates may be stored at 2-8°C for up to 6 months.
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Product Size: 1 L
Reagent Type: Blocking Reagent
Validated Application: ELISA

Contents & storage

Store at 2–8°C.


Manuals & protocols