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Rat Glial Precursor Cells - FAQs

View additional product information for Rat Glial Precursor Cells - FAQs (N7746100)

6 product FAQs found

How long can I culture Gibco Rat Glial Precursor Cells?

These cells can be thawed and passaged once before using in experiments. The single passage will yield a 2-fold increase of thawed cells. Cells do not expand significantly beyond first passage post-thaw.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where were Gibco Rat Glial Precursor Cells (Cat. No. N7746100) isolated from? How many passages do these cells go through before being cryopreserved?

Rat Glial Precursor Cells were isolated from the cortex of new born Sprague Dawley rats at day 2 after birth, and cryopreserved at passage 2.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the growth factors or cytokines used in neural stem cell (NSC) culture?

For NSC expansion, the following growth factors are used: recombinant EGF (Cat. No. PHG0314), recombinant FGF-basic (Cat. No. PHG0024), and recombinant VEGF (Cat. No. PHC9394). In addition, several neurotrophins such as BDNF Cat. No. 10908010), NT-3, CNTF (Cat. No. PHC7015), and GDNF (Cat. No. PHC7044)are also used in the related studies.

Information pertaining to whether a specific product has been tested against the WHO Reference Standard can typically be located on the product page or Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture human neural stem cells (NSCs)?

Human NSCs can grow in Gibco StemPro NSC SFM (Cat. No. A1050901) on dishes pre-coated with Gibco Geltrex Matrix or Gibco CELLstart substrate. Alternatively, if the goal is to obtain neurons, NSCs can also be grown on Neurobasal medium supplemented with Gibco B-27 supplements without vitamin A on a pre-coated dish.

How can I characterize neural stem cells (NSCs)?

NSCs are generally characterized by their ability to form neurospheres when plated at cloning density (Nat Methods 2:333 (2005)). NSCs can also be characterized by (1) RT-PCR of Sox1, Sox2, and Nestin or (2) immunohistochemical staining for nestin, Pax6, Sox2, and Ki67.

What are neural stem cells?

Neural stem cells (NSCs) are self-renewing multipotent cells of the nervous system capable of differentiating into neurons, oligodendrocytes, and astrocytes. NSC can be generated by induced differentiation from embryonic stem (ES) cells, or isolated from various regions of the brain including the cortex, the subventricular zone (SVZ), and the ventricular zone, or generated from bone marrow-derived mesenchymal stem cells (MSCs) (J Cell Biochem 114:764 (2013)). NSCs are valuable tools for the study of neurogenesis and neurotransmitter and receptor function. NSCs were used in the investigation of different CNS disorders such as PD and Huntington's disease in various animal models (J Cell Biochem 114:764 (2013)).