Nitric oxide protects cultured rat hepatocytes from tumor necrosis factor-alpha-induced apoptosis by inducing heat shock protein 70 expression.
AuthorsKim YM,de Vera ME,Watkins SC,Billiar TR
JournalThe Journal of biological chemistry
PubMed ID8995451
Arginase II downregulates nitric oxide (NO) production and prevents NO-mediated apoptosis in murine macrophage-derived RAW 264.7 cells.
AuthorsGotoh T, Mori M
JournalJ Cell Biol
PubMed ID9971738
'Excess nitric oxide (NO) induces apoptosis of some cell types, including macrophages. As NO is synthesized by NO synthase (NOS) from arginine, a common substrate of arginase, these two enzymes compete for arginine. There are two known isoforms of arginase, types I and II. Using murine macrophage-like RAW 264.7 cells, ... More
Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells.
AuthorsGarg UC, Hassid A
JournalJ Clin Invest
PubMed ID2540223
'Endothelium-derived relaxing factor has been recently identified as nitric oxide. The purpose of this study was to determine if vasodilator drugs that generate nitric oxide inhibit vascular smooth muscle mitogenesis and proliferation in culture. Three chemically dissimilar vasodilators, sodium nitroprusside, S-nitroso-N-acetylpenicillamine and isosorbide dinitrate, dose-dependently inhibited serum-induced thymidine incorporation by ... More
Phosphorylation of the inositol 1,4,5-trisphosphate receptor. Cyclic GMP-dependent protein kinase mediates cAMP and cGMP dependent phosphorylation in the intact rat aorta.
AuthorsKomalavilas P, Lincoln TM
JournalJ Biol Chem
PubMed ID8702997
'The effects of cyclic GMP (cGMP) and activation of cGMP-dependent protein kinase (PKG) on the phosphorylation of the inositol 1,4, 5-trisphosphate (IP3) receptor were examined in intact rat aorta using the technique of back phosphorylation. Aorta treated with the nitric oxide donors, S-nitroso-N-acetylpenicillamine and sodium nitroprusside, or the selective PKG ... More
Modulation of endogenous antioxidant enzymes by nitric oxide in rat C6 glial cells.
AuthorsDobashi K, Pahan K, Chahal A, Singh I
JournalJ Neurochem
PubMed ID9109515
'To understand the possible mechanism of nitric oxide (NO)-mediated cytotoxicity, we investigated the effect of NO on the endogenous antioxidant enzymes (AOEs) catalase, glutathione peroxidase (GPX), and CuZn- and Mn-superoxide dismutases (SODs) in rat C6 glial cells under conditions in which these cells expressed oligodendrocyte-like properties as evidenced by the ... More
Role of glutathione in nitric oxide-mediated injury to rat gastric mucosal cells.
AuthorsWakulich CA, Tepperman BL
JournalEur J Pharmacol
PubMed ID9042609
'Recent studies suggest that in some cell types, the activity of nitric oxide (NO) is influenced by the endogenous antioxidant, reduced glutathione (GSH). The present study has examined the role of GSH in NO-induced cytotoxicity in cells harvested from the rat gastric mucosa. Cell integrity was assessed by Trypan blue ... More
Ca(2+)-mediated damage to rabbit gastric mucosal cells: modulation by nitric oxide.
AuthorsTepperman BL, Soper BD
JournalEur J Pharmacol
PubMed ID8666044
'Pertubations in cellular Ca2+ homeostasis can lead to oxidative stress whereas nitric oxide has been shown to inactivate oxygen radicals. Therefore the effects of inhibition of nitric oxide (NO) synthase activity on Ca2+-mediated disruption to rabbit dispersed gastric mucosal cells have been examined. Addition of the Ca2+ ionophore A23187 (3-25 ... More
Inhibition of excitatory amino acid-induced phosphoinositide hydrolysis as a possible mechanism of nitroprusside neurotoxicity.
AuthorsYu O, Chuang DM
JournalJ Neurochem
PubMed ID8522973
'Inclusion of sodium nitroprusside (Na2[Fe(2+)-(CN)5NO]) into the culture medium is toxic to cultured rat cerebellar granule neurons. A possible underlying mechanism may be the inhibition of phosphoinositide (PI) response to excitatory amino acids (EAAs) because activation of glutamate receptors can be neuroprotective and neurotrophic in differentiating neurons. Sodium nitroprusside selectively ... More
Reversible S-nitrosation and inhibition of HIV-1 protease.
AuthorsSehajpal PK, Basu A, Ogiste JS, Lander HM
JournalBiochemistry
PubMed ID10529217
'Nitric oxide (*NO) is a short-lived free radical with many functions including vasoregulation, synaptic plasticity, and immune modulation and has recently been associated with AIDS pathology. Various pathophysiological conditions, such as viral infection, trigger inducible nitric oxide synthase (iNOS) to synthesize NO in the cell. NO-derived species can react with ... More
Activation of p70 S6 kinase and erk-encoded mitogen-activated protein kinases is resistant to high cyclic nucleotide levels in Swiss 3T3 fibroblasts.
'Treatment of Swiss mouse 3T3 fibroblasts with certain cyclic nucleotide phosphodiesterase inhibitors (theophylline, SQ 20,006, and MY-5445) prevents the activation of the M(r) 70,000 S6 kinase (p70S6k) induced by a variety of external stimuli. Concentrations giving half-maximal inhibition were 800, 50, and 25 microM, respectively. Western blot analysis and immunocomplex ... More
The oxidation of alpha-tocopherol in human low-density lipoprotein by the simultaneous generation of superoxide and nitric oxide.
AuthorsHogg N, Darley-Usmar VM, Wilson MT, Moncada S
JournalFEBS Lett
PubMed ID8391994
'Peroxynitrite is the product of the reaction between nitric oxide and superoxide. It is an oxidant which can also decompose to form the hydroxyl radical and nitrogen dioxide. In this report we show that a powerful oxidant with reactivity similar to that of the hydroxyl radical is formed from the ... More
Koningic acid (a potent glyceraldehyde-3-phosphate dehydrogenase inhibitor)-induced fragmentation and condensation of DNA in NG108-15 cells.
AuthorsNakazawa M, Uehara T, Nomura Y
JournalJ Neurochem
PubMed ID9166744
'We examined nitric oxide (NO)-induced cell death in NG108-15 cells using NO donors. Both sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine caused lactate dehydrogenase (LDH) leakage from NG108-15 cells. NO is known to increase the amount of radioisotopic labeled glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the presence of [32P]NAD and to inhibit the enzyme ... More
Nitric oxide disrupts Ca2+ homeostasis in hippocampal neurons.
AuthorsBrorson JR, Sulit RA, Zhang H
JournalJ Neurochem
PubMed ID8978714
'Nitric oxide has been recognized in recent years as an important mediator of neuronal toxicity, which in many cases involves alterations of the cytoplasmic Ca2+ concentration ([Ca2+]i). In [Ca2+]i fluorimetric experiments on cultured hippocampal neurons, the nitric oxide-releasing agent S-nitrosocysteine produced a delayed rise in [Ca2+]i over a 20-min exposure, ... More
Poly(ADP-ribose) synthetase activation mediates mitochondrial injury during oxidant-induced cell death.
AuthorsVirág L, Salzman AL, Szabó C
JournalJ Immunol
PubMed ID9759901
'Reactive oxidant species are important mediators of tissue injury in shock, inflammation, and reperfusion injury. The actions of a number of these oxidants (e.g., hydroxyl radical and peroxynitrite, a reactive oxidant produced by the reaction of nitric oxide and superoxide) are mediated in part by the activation of the nuclear ... More
Inhibitory effect of nitric oxide on the replication of a murine retrovirus in vitro and in vivo.
AuthorsAkarid K, Sinet M, Desforges B, Gougerot-Pocidalo MA
JournalJ Virol
PubMed ID7474119
'Nitric oxide (NO) exerts microbicidal effects on a broad spectrum of pathogens, including viruses, but its antiretrovirus properties have not yet been described. The purpose of this study was to determine whether NO inhibits murine Friend leukemia virus (FV) replication in vitro and to what extent NO may play a ... More
Characterization of sulfur-centered radical intermediates formed during the oxidation of thiols and sulfite by peroxynitrite. ESR-spin trapping and oxygen uptake studies.
AuthorsKaroui H, Hogg N, Fréjaville C, Tordo P, Kalyanaraman B
JournalJ Biol Chem
PubMed ID8626383
'Using a novel phosphorylated spin trap, 5-diethoxy-phosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), an analog of the commonly used trap 5,5''-dimethyl-1-pyrroline N-oxide (DMPO), we have investigated the reactions of sulfur-centered radicals produced from the oxidation of thiols and sulfite by peroxynitrite. The predominant species trapped in all cases are the corresponding sulfur-centered radicals, i.e. ... More
Behavioral role for nitric oxide in chemosensory activation of feeding in a mollusc.
AuthorsElphick MR, Kemenes G, Staras K, O'Shea M
JournalJ Neurosci
PubMed ID7472516
'A role for the NO-cGMP pathway in mediating chemosensory activation of feeding is suggested by intense NADPH diaphorase staining observed in nerve fibers that project from sensory cells in the lips to the CNS and by the presence in the CNS of a NO-activated guanylyl cyclase. In preparations reduced to ... More
Endothelin-1 production by rat inner medullary collecting duct: effect of nitric oxide, cGMP, and immune cytokines.
AuthorsKohan DE, Padilla E
JournalAm J Physiol
PubMed ID8141330
'Nitric oxide (NO), guanosine 3'',5''-cyclic monophosphate (cGMP), and endothelin-1 (ET-1) inhibit collecting duct sodium reabsorption. Because the inner medullary collecting duct (IMCD) synthesizes NO and ET-1, we examined NO and cGMP regulation of IMCD ET-1 production. S-nitroso-N-acetylpenicillamine (SNAP, 6 h) increased NO and cGMP and modestly reduced ET-1 release in ... More
Lack of tolerance to a 24-hour infusion of S-nitroso N-acetylpenicillamine (SNAP) in conscious rabbits.
AuthorsShaffer JE, Han BJ, Chern WH, Lee FW
JournalJ Pharmacol Exp Ther
PubMed ID1731043
'In order to design a means to preclude the development of nitrate tolerance, an in vivo model of nitroglycerin (GTN) tolerance was developed in the rabbit to examine the tolerance potential of S-nitroso vasodilators. Three separate studies were performed with 54 male New Zealand white rabbits which were instrumented for ... More
Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite-mediated reperfusion injury.
AuthorsWang P, Zweier JL
JournalJ Biol Chem
PubMed ID8910581
'Altered nitric oxide (NO.) production is a critical factor in tissue reperfusion injury; however, controversy remains regarding these alterations and how they cause injury. Since superoxide (O-2) generation is triggered during the early period of reperfusion the cytotoxic oxidant peroxynitrite (ONOO-) could be formed, but it is not known if ... More
Modulation of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) binding sites by nitric oxide.
AuthorsDev KK, Morris BJ
JournalJ Neurochem
PubMed ID7519666
'Nitric oxide release is reported to be involved in physiological processes associated with altered sensitivity of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) class of glutamate receptor. A series of compounds liberating nitric oxide were therefore tested for their ability to modulate in vitro the characteristics of [3H]AMPA binding to sections of rat ... More
Biochemical characterization of human S-nitrosohemoglobin. Effects on oxygen binding and transnitrosation.
AuthorsPatel RP, Hogg N, Spencer NY, Kalyanaraman B, Matalon S, Darley-Usmar VM
JournalJ Biol Chem
PubMed ID10336440
'S-Nitrosation of cysteine beta93 in hemoglobin (S-nitrosohemoglobin (SNO-Hb)) occurs in vivo, and transnitrosation reactions of deoxygenated SNO-Hb are proposed as a mechanism leading to release of NO and control of blood flow. However, little is known of the oxygen binding properties of SNO-Hb or the effects of oxygen on transnitrosation ... More
Endothelin-stimulated capacitative calcium entry in enteric glial cells: synergistic effects of protein kinase C activity and nitric oxide.
'Depletion of intracellular calcium stores by agonist stimulation is coupled to calcium influx across the plasma membrane, a process termed capacitative calcium entry. Capacitative calcium entry was examined in cultured guinea pig enteric glial cells exposed to endothelin 3. Endothelin 3 (10 nM) caused mobilization of intracellular calcium stores followed ... More
A mitogen-activated protein kinase-dependent signaling pathway in the activation of platelet integrin alpha IIbbeta3.
AuthorsLi Z, Xi X, Du X
JournalJ Biol Chem
PubMed ID11522789
'We have recently shown that the platelet integrin alpha(IIb)beta(3) is activated by von Willebrand factor (vWF) binding to its platelet receptor, glycoprotein Ib-IX (GPIb-IX), via the protein kinase G (PKG) signaling pathway. Here we show that GPIb-IX-mediated activation of integrin alpha(IIb)beta(3) is inhibited by dominant negative mutants of Raf-1 and ... More
Metallothionein protects against the cytotoxic and DNA-damaging effects of nitric oxide.
AuthorsSchwarz MA, Lazo JS, Yalowich JC, Allen WP, Whitmore M, Bergonia HA, Tzeng E, Billiar TR, Robbins PD, Lancaster JR
JournalProc Natl Acad Sci U S A
PubMed ID7538671
'In inflammatory states, nitric oxide (.NO) may be synthesized from precursor L-arginine via inducible .NO synthase (iNOS) in large amounts for prolonged periods of time. When .NO acts as an effector molecule under these conditions, it may be toxic to cells by inhibition of iron-containing enzymes or initiation of DNA ... More
Nitric oxide regulates IL-8 expression in melanoma cells at the transcriptional level.
AuthorsAndrew PJ, Harant H, Lindley IJ
JournalBiochem Biophys Res Commun
PubMed ID7575568
'We investigated the role of nitric oxide (NO) in the expression of interleukin-8 (IL-8) in the human melanoma cell line, G361. Three NO donors, 3-morpholinosydnonimine hydrochloride (SIN-1), S-nitroso-N-acetylpenicillamine (SNAP), and S-nitroso-L-glutathione (SNOG), all caused an increase in both IL-8 protein secretion and promoter activity. Truncation of the promoter showed that ... More
Nitric oxide donation and nitrite assays in the presence of thiols and albumin as determined by Griess' and Werringloer's methods.
AuthorsRobak J, Marcinkiewicz E, Michalska Z, Gryglewski RJ
JournalPol J Pharmacol
PubMed ID9437769
'Nitric oxide (NO) or nitrite (NO2-) were assayed using the Werringloer''s method or the Griess'' method, respectively, in the presence or absence of various thiols, amino acids, or albumin. This has been done because both methods are used to determine the generation of endogenous NO from L-arginine or exogenous NO ... More
Modulation by nitric oxide of rat brain GABAA receptors.
AuthorsZarri I, Bucossi G, Cupello A, Rapallino MV, Robello M
JournalNeurosci Lett
PubMed ID7535408
'The effect of nitric oxide (NO) on the function of GABAA receptors was studied in two different rat brain neuron populations. Cerebral cortex neuronal GABAA receptors were studied by preparing microsacs and evaluating 36Cl- accumulation. Whether nitric oxide was provided by sodium nitroprusside (SNP) or by the metabolic precursor precursor ... More
Differential induction of apoptosis in Swiss 3T3 cells by nitric oxide and the nitrosonium cation.
AuthorsKhan S, Kayahara M, Joashi U, Mazarakis ND, Sarraf C, Edwards AD, Hughes MN, Mehmet H
JournalJ Cell Sci
PubMed ID9378780
'We have investigated the effect of nitric oxide (NO) on apoptosis in Swiss 3T3 fibroblasts and compared it to the effect of the nitrosonium cation (NO+). Both species induced apoptosis, confirmed by electron microscopy, propidium iodide staining, DNA laddering and activation of caspases. The kinetics of triggering apoptosis were different ... More
Nitric oxide generators and cGMP stimulate mucus secretion by rat gastric mucosal cells.
AuthorsBrown JF, Keates AC, Hanson PJ, Whittle BJ
JournalAm J Physiol
PubMed ID8214062
'The effect of nitric oxide (NO) donors on the release of mucus from a suspension of isolated gastric cells was investigated by using an enzyme-linked immunosorbent assay for rat gastric mucin. Isosorbide dinitrate (ISDN, 0.1-2 mM) produced a dose-related stimulation of mucus secretion, without affecting the viability of the isolated ... More
S100beta induces neuronal cell death through nitric oxide release from astrocytes.
AuthorsHu J, Ferreira A, Van Eldik LJ
JournalJ Neurochem
PubMed ID9375660
'The glial-derived neurotrophic protein S100beta has been implicated in the development and maintenance of the nervous system. S100beta has also been postulated to play a role in mechanisms of neuropathology because of its specific localization and selective overexpression in Alzheimer''s disease. However, the exact relationship between S100beta overexpression and neurodegeneration ... More
Differential properties of two gap junctional pathways made by AII amacrine cells.
AuthorsMills SL, Massey SC
JournalNature
PubMed ID7477263
'The retina is sensitive to light stimuli varying over more than 12 log units in intensity. It accomplishes this, in part, by switching between rod-dominated circuits designed for maximum utilization of scarce photons and cone circuits designed for greater acuity. Rod signals are integrated into the cone pathways through AII ... More
Effect of nitric oxide on mitogenesis and proliferation of cerebellar glial cells.
AuthorsGarg UC, Devi L, Turndorf H, Goldfrank LR, Bansinath M
JournalBrain Res
PubMed ID1333340
'In the brain, nitric oxide (NO) has been identified as a messenger molecule and a mediator of excitatory amino acid-induced neurotoxicity. In this study, the effects of NO on serum-induced mitogenesis and cell proliferation of the cerebellar glial cells were assessed. NO-generating agent, S-nitroso-N-acetylpenicillamine (SNAP) increased intracellular cyclic guanosine monophosphate ... More
Direct measurement of nitric oxide generation in the ischemic heart using electron paramagnetic resonance spectroscopy.
AuthorsZweier JL, Wang P, Kuppusamy P
JournalJ Biol Chem
PubMed ID7814391
'Nitric oxide, NO., exerts numerous important regulatory functions in biological tissues and has been hypothesized to have a role in the pathogenesis of cellular injury in a number of diseases. It has been suggested that alterations in NO. generation are a critical cause of injury in the ischemic heart. However, ... More
Nitric oxide regulates interleukin 1 bioactivity released from murine macrophages.
AuthorsHill JR, Corbett JA, Kwon G, Marshall CA, McDaniel ML
JournalJ Biol Chem
PubMed ID8798439
'The bioactivity of interleukin-1 (IL-1), a major proinflammatory cytokine, can be modulated by a variety of factors including inhibitors of IL-1 production and release and receptor blockade by IL-1 receptor antagonist and by binding to nonsignaling soluble receptors. This study demonstrates that the free radical nitric oxide (NO) is also ... More
Nitric oxide stimulates tyrosine phosphorylation in murine fibroblasts in the absence and presence of epidermal growth factor.
AuthorsPeranovich TM, da Silva AM, Fries DM, Stern A, Monteiro HP
JournalBiochem J
PubMed ID7530447
'In the present study, utilizing anti-phosphotyrosine monoclonal antibodies, sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) as sources of NO and murine fibroblasts expressing the human epidermal growth factor (EGF) receptor (HER14 cells), we showed that tyrosine phosphorylation of a set of proteins (126, 56 and 43 kDa) was stimulated when cells ... More
Nitric oxide-induced apoptosis in RAW 264.7 macrophages is antagonized by protein kinase C- and protein kinase A-activating compounds.
AuthorsMessmer UK, Lapetina EG, Brüne B
JournalMol Pharmacol
PubMed ID7723736
'Endogenously generated or exogenously applied nitric oxide (NO) redox species induce apoptotic cell death in murine RAW 264.7 macrophages. Activation of the inducible NO synthase by incubation of cells with a combination of lipopolysaccharide and interferon-gamma produced internucleosomal DNA fragmentation and morphological alterations, i.e., chromatin condensation, indicative of apoptotic cell ... More
Mercaptoethylguanidine and guanidine inhibitors of nitric-oxide synthase react with peroxynitrite and protect against peroxynitrite-induced oxidative damage.
AuthorsSzabó C, Ferrer-Sueta G, Zingarelli B, Southan GJ, Salzman AL, Radi R
JournalJ Biol Chem
PubMed ID9083027
'Nitric oxide (NO) produced by the inducible nitric-oxide synthase (iNOS) is responsible for some of the pathophysiological alterations during inflammation. Part of NO-related cytotoxicity is mediated by peroxynitrite, an oxidant species produced from NO and superoxide. Aminoguanidine and mercaptoethylguanidine (MEG) are inhibitors of iNOS and have anti-inflammatory properties. Here we ... More
AuthorsTrotti D, Rossi D, Gjesdal O, Levy LM, Racagni G, Danbolt NC, Volterra A
JournalJ Biol Chem
PubMed ID8626378
'The reuptake of glutamate in neurons and astrocytes terminates excitatory signals and prevents the persistence of excitotoxic levels of glutamate in the synaptic cleft. This process is inhibited by oxygen radicals and hydrogen peroxide (H2O2). Here we show that another biological oxidant, peroxynitrite (ONOO-), formed by combination of superoxide (O2-) ... More
Constitutive and permissive roles of nitric oxide activity in embryonic ciliary cells.
AuthorsDoran SA, Tran CH, Eskicioglu C, Stachniak T, Ahn KC, Goldberg JI
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID12676758
'Embryos of Helisoma trivolvis exhibit cilia-driven rotation within the egg capsule during development. In this study we examined whether nitric oxide (NO) is a physiological regulator of ciliary beating in cultured ciliary cells. The NO donor S-nitroso-N-acetylpenicillamine (SNAP; 1-1,000 microM) produced a dose-dependent increase in ciliary beat frequency (CBF). In ... More
'To examine the role of nitric oxide (.NO) in vascular endothelial cell injury, cultured porcine pulmonary artery endothelial cells (PAEC) were treated with H2O2 (100-500 microM) for 30 min in the presence or absence of the .NO donors (+/-)S-nitroso-N-acetylpenicillamine (SNAP) or diethylamine nitric oxide (DEANO). H2O2 caused dose-dependent PAEC cytotoxicity ... More
Involvement of L-type-like amino acid transporters in S-nitrosocysteine-stimulated noradrenaline release in the rat hippocampus.
AuthorsSatoh S, Kimura T, Toda M, Maekawa M, Ono S, Narita H, Miyazaki H, Murayama T, Nomura Y
JournalJ Neurochem
PubMed ID9349567
'Nitrogen oxides, such as nitric oxide, have been shown to regulate neuronal functions, including neurotransmitter release. We investigated the effect of S-nitroso-L-cysteine (SNC) on noradrenaline (NA) release in the rat hippocampus in vivo and in vitro. SNC stimulated [3H]NA release from prelabeled hippocampal slices in a dose-dependent manner. SNC stimulated ... More
Ultraviolet B radiation acts through the nitric oxide and cGMP signal transduction pathway to stimulate melanogenesis in human melanocytes.
AuthorsRoméro-Graillet C, Aberdam E, Biagoli N, Massabni W, Ortonne JP, Ballotti R
JournalJ Biol Chem
PubMed ID8910416
'Ultraviolet B (UVB) radiation is the main physiological stimulus for human skin pigmentation; however, the molecular mechanisms underlying this process are still unclear. Recently, nitric oxide (NO) and cGMP have been involved in mediation of skin erythema induced by UVB. Therefore, we investigated the role of NO and cGMP in ... More
S-nitroso-glutathione inhibits platelet activation in vitro and in vivo.
AuthorsRadomski MW, Rees DD, Dutra A, Moncada S
JournalBr J Pharmacol
PubMed ID1335336
'1. The effect of S-nitroso-glutathione (GSNO), a stable nitrosothiol, on platelet activation was examined in vitro and in vivo. 2. The adhesion of human platelets to fibrillar collagen and human endothelial cell monolayers was inhibited by GSNO. 3. GSNO caused a concentration-dependent inhibition of collagen-induced platelet aggregation in vitro and ... More
Elevated production of salivary nitric oxide in oral mucosal diseases.
AuthorsOhashi M, Iwase M, Nagumo M
JournalJ Oral Pathol Med
PubMed ID10478960
'Nitric oxide (NO) is known to play an important role in biological systems. In this study, we measured levels of NO in the saliva of 39 patients with oral mucosal diseases: 21 had oral lichen planus (OLP) and 18 had recurrent aphthous ulceration (RAU). NO was assayed using the Griess ... More
LTRPC2 Ca2+-permeable channel activated by changes in redox status confers susceptibility to cell death.
AuthorsHara Y, Wakamori M, Ishii M, Maeno E, Nishida M, Yoshida T, Yamada H, Shimizu S, Mori E, Kudoh J, Shimizu N, Kurose H, Okada Y, Imoto K, Mori Y
JournalMol Cell
PubMed ID11804595
'Redox status changes exert critical impacts on necrotic/apoptotic and normal cellular processes. We report here a widely expressed Ca2+-permeable cation channel, LTRPC2, activated by micromolar levels of H2O2 and agents that produce reactive oxygen/nitrogen species. This sensitivity of LTRPC2 to redox state modifiers was attributable to an agonistic binding of ... More
Nitric oxide: a new paradigm for second messengers.
AuthorsKerwin JF, Lancaster JR, Feldman PL
JournalJ Med Chem
PubMed ID7473563
The kinetics of S-transnitrosation--a reversible second-order reaction.
AuthorsHogg N
JournalAnal Biochem
PubMed ID10415097
Transnitrosation between thiols and S-nitrosothiols has been suggested to be a mechanism of signal transduction. This kinetics of such reactions fit well to a reversible second-order model. Parameters derived from this model give both the forward and reverse rate constants and the equilibrium position at physiological temperature and pH. In ... More
Toxicity of chemically generated nitric oxide towards pancreatic islet cells can be prevented by nicotinamide.
AuthorsKallmann B, Burkart V, Kröncke KD, Kolb-Bachofen V, Kolb H
JournalLife Sci
PubMed ID1386894
Previous studies have indicated that nitric oxide is involved in the lysis of pancreatic islet cells by inflammatory macrophages. Here we show that the incubation of islet cells with chemical NO-donors leads to cell lysis in a concentration and time dependent way. Islet cell death could be prevented by nicotinamide ... More
Tolerance to relaxation in rat aorta: comparison of an S-nitrosothiol with nitroglycerin.
AuthorsKowaluk EA, Poliszczuk R, Fung HL
JournalEur J Pharmacol
PubMed ID3126076
Tolerance and cross-tolerance to the relaxant effects of S-nitroso-N-acetylpenicillamine (SNAP) and nitroglycerin were examined in rat aortic rings. Nitroglycerin-tolerant rings remained fully responsive to SNAP and sodium nitroprusside. Thus, reduced metabolic activation of nitroglycerin, rather than impaired guanylate cyclase activity, appeared to be the operating mechanism for nitrate tolerance in ... More
Mitochondrial biogenesis in mammals: the role of endogenous nitric oxide.
AuthorsNisoli E, Clementi E, Paolucci C, Cozzi V, Tonello C, Sciorati C, Bracale R, Valerio A, Francolini M, Moncada S, Carruba MO
JournalScience
PubMed ID12574632
Nitric oxide was found to trigger mitochondrial biogenesis in cells as diverse as brown adipocytes and 3T3-L1, U937, and HeLa cells. This effect of nitric oxide was dependent on guanosine 3',5'-monophosphate (cGMP) and was mediated by the induction of peroxisome proliferator-activated receptor gamma coactivator 1alpha, a master regulator of mitochondrial ... More
Control of NO concentration in solutions of nitrosothiol compounds by light.
AuthorsZhelyaskov VR, Gee KR, Godwin DW
JournalPhotochem Photobiol
PubMed ID9523529
We studied the thermal and photolytic decomposition of two S-nitrosothiols, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylpenicillamine (SNAP), in water or propanol solutions. A "concentration clamp" (relatively constant concentration of NO as a function of time) could be implemented in a closed volume by varying the pH, concentration of nitrovasodilator and intensity of ... More
Interaction between endothelium-derived relaxing factors, S-nitrosothiols, and endothelin-1 on Ca2+ mobilization in rat vascular smooth muscle cells.
AuthorsOkishio M, Ohkawa S, Ichimori Y, Kondo K
JournalBiochem Biophys Res Commun
PubMed ID1550591
S-Nitrosothiols (S-nitrosocysteine, S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine), which belong to the group of endothelium-derived relaxing factors (EDRFs), caused decreases of cytosolic free Ca2+ concentrations ([Ca2+]i) in cultured rat vascular smooth muscle cells (VSMCs). The endothelin-1 (ET-1)-induced sustained increase of [Ca2+]i in rat VSMCs was completely abolished by preaddition of at least an ... More
Effects of nitric oxide on glutamate-gated channels and other ionic channels.
AuthorsFagni L, Bockaert J
JournalJ Chem Neuroanat
PubMed ID8811428
Nitric oxide is an endogenous molecule that plays a role of second messenger in the central and peripheral nervous system. A major action of this molecule is to control ionic channel activity. Because of technical difficulties to use nitric oxide as a gaseous compound, nitric oxide donors are often utilized ... More
S-nitrosothiol inhibition of mitochondrial complex I causes a reversible increase in mitochondrial hydrogen peroxide production.
AuthorsBorutaite V, Brown GC
JournalBiochim Biophys Acta
PubMed ID16584707
We found that reversible inactivation of mitochondrial complex I by S-nitroso-N-acetyl-D,L-penicillamine (SNAP) in isolated rat heart mitochondria resulted in a three-fold increase in H2O2 production, when mitochondria were respiring on pyruvate and malate, (but not when respiring on succinate or in the absence of added respiratory substrate). The inactivation of ... More
Loss and degradation of enzyme-bound heme induced by cellular nitric oxide synthesis.
AuthorsKim YM, Bergonia HA, Müller C, Pitt BR, Watkins WD, Lancaster JR
JournalJ Biol Chem
PubMed ID7890697
We report here that, like nonheme iron, protein-bound intracellular heme iron is also a target for destruction by endogenously produced nitric oxide (NO). In isolated rat hepatocytes NO synthesis results in substantial (approximately 60%) and comparable loss of catalase and cytochrome P450 as well as total microsomal heme, and decreased ... More
Inactivation of brain tryptophan hydroxylase by nitric oxide.
AuthorsKuhn DM, Arthur RE
JournalJ Neurochem
PubMed ID8752114
Tryptophan hydroxylase, the initial and rate-limiting enzyme in the biosynthesis of the neurotransmitter serotonin, is inactivated by nitric oxide (NO) and by the NO generators sodium nitroprusside, diethylamine/NO, S-nitroso-N-acetylpenicillamine, and S-nitrosocysteine. The inactivation occurs in an oxygen-free environment and is enhanced by dithiothreitol and ascorbic acid. Protection against the effect ... More
Adenylyl cyclase, a coincidence detector for nitric oxide.
AuthorsMcVey M, Hill J, Howlett A, Klein C
JournalJ Biol Chem
PubMed ID10383385
Nitric oxide (NO) donors inhibit hormone- and forskolin-stimulated adenylyl cyclase activity in purified plasma membrane preparations from N18TG2 neuroblastoma cells. Northern blot analyses indicate that the predominant isoform of adenylyl cyclase in N18TG2 cells is the type VI. Our experiments eliminate all the known regulatory proteins for this isoform as ... More
The nitric oxide donor, S-nitroso-N-acetyl-penicillamine, inhibits secretory activity in rat isolated parietal cells.
AuthorsBrown JF, Hanson PJ, Whittle BJ
JournalBiochem Biophys Res Commun
PubMed ID8216268
Accumulation of the weak base aminopyrine was used as an index of acid secretory activity in rat isolated parietal cells. The nitric oxide (NO) donor, S-nitroso-N-acetyl-penicillamine (SNAP) caused a dose-dependent inhibition of aminopyrine accumulation (half-maximally effective concentration 247 microM) which was accompanied by an increase in guanosine 3',5'-cyclic monophosphate (cGMP) ... More
Simultaneous measurement of intracellular Ca(2+) and nitric oxide: a new method.
AuthorsBerkels R, Dachs C, Roesen R, Klaus W
JournalCell Calcium
PubMed ID10859594
Different methods to measure the unstable radical nitric oxide (NO) have been established. We are going to present a new method to measure intracellular calcium and NO simultaneously in endothelial cells. A new fluorescent dye (DAF-2) has been developed recently which binds NO resulting in an enhanced fluorescence. We loaded ... More
Nitric oxide-induced [3H] GABA release from cerebral cortical neurons is mediated by peroxynitrite.
AuthorsOhkuma S, Narihara H, Katsura M, Hasegawa T, Kuriyama K
JournalJ Neurochem
PubMed ID7643088
The functional significance of peroxynitrite in the release of [3H]GABA induced by nitric oxide (NO) liberated from NO generators was investigated using cerebral cortical neurons in primary culture. NO generators such as sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) increased [3H] GABA release in a dose-dependent manner. These increases in [3H]-GABA ... More
Conversion of nitroxyl (HNO) to nitric oxide (NO) in biological systems: the role of physiological oxidants and relevance to the biological activity of HNO.
AuthorsFukuto JM, Hobbs AJ, Ignarro LJ
JournalBiochem Biophys Res Commun
PubMed ID8240347
Nitroxyl (HNO) and nitric oxide (NO) are chemically related compounds in that NO is the one-electron oxidation product of HNO. Previous studies from this laboratory indicated that HNO elicits pharmacological effects that are similar to those elicited by NO, namely, vascular smooth muscle relaxation and stimulation of cyclic GMP formation. ... More
Generation of nitric oxide from S-nitrosothiols using protein-bound Cu2+ sources.
AuthorsDicks AP, Williams DL
JournalChem Biol
PubMed ID8807899
BACKGROUND: We have recently shown that S-nitrosothiols (RSNOs) decompose in aqueous buffer to give nitric oxide, an important signalling molecule, and the corresponding disulphides. This occurs by reaction with Cu+ generated from Cu2+ (supplied as hydrated Cu2+) by thiolate reduction. To establish whether these reactions are feasible in vivo, we ... More
Macrophage-derived nitric oxide regulates T cell activation via reversible disruption of the Jak3/STAT5 signaling pathway.
AuthorsBingisser RM, Tilbrook PA, Holt PG, Kees UR
JournalJ Immunol
PubMed ID9637481
Nitric oxide (NO) has been invoked as an important pathogenic factor in a wide range of immunologically mediated diseases. The present study demonstrates that macrophage-derived NO may conversely function to fine tune T cell-mediated inflammation via reversible dephosphorylation of intracellular signaling molecules, which are involved in the control of T ... More
Tumor necrosis factor-alpha plus actinomycin D-induced apoptosis of L929 cells is prevented by nitric oxide.
AuthorsHakoda S, Ishikura H, Takeyama N, Tanaka T
JournalSurg Today
PubMed ID10554331
Treatment with the nitric oxide-(NO)-generating compound S-nitroso-N-acetylpenicillamine protected cul-tured L929 cells from apoptosis induced by tumor necrosis factor-alpha (TNF-alpha) plus actinomycin D, as determined by the detection of DNA fragmentation and morphological changes. NO also prevented an enhancement of the production of reactive oxygen intermediates by TNF-alpha plus actinomycin D, ... More
Stimulation of immediate early gene expression in striatal neurons by nitric oxide.
AuthorsMorris BJ
JournalJ Biol Chem
PubMed ID7559590
Exposure of primary cultures of embryonic rat striatal neurons to agents releasing nitric oxide (NO), including sin-1 molsidomine, S-nitroso-n-acetyl-penicillamine (SNAP), and S-nitrosoglutathione, resulted in an increase in the levels of expression of the immediate early genes c-fos and zif/268 in the cultured neurons. The membrane-permeable cGMP analogue, 8-bromo-cGMP, did not ... More
Peroxynitrite leads to arteriolar smooth muscle cell membrane hyperpolarization and low vasoreactivity in severe shock.
AuthorsZhao KS, Liu J, Yang GY, Jin C, Huang Q, Huang X
JournalClin Hemorheol Microcirc
PubMed ID11321449
This paper aimed to study the mechanism of vascular hyporeactivity during severe hemorrhagic shock. Rats were divided into control and shock group. Membrane potential of arteriolar strips was measured with intracellular recording method and membrane potential changes in arteriolar smooth muscle cells (ASMC) were recorded with membrane potential sensitive fluorescent ... More
Mechanisms of nitrosothiol-induced antimitogenesis in aortic smooth muscle cells.
AuthorsGarg UC, Hassid A
JournalEur J Pharmacol
PubMed ID7689974
The purpose of this work was to investigate the effect of a nitrosothiol vasodilator, S-nitroso-N-acetylpenicillamine (SNAP), on serum-induced cell proliferation, thymidine uptake, RNA synthesis, protein synthesis and thymidine kinase activity in a cultured rat aortic smooth muscle cell line. SNAP decreased the rate of serum-stimulated cell proliferation, thymidine uptake and ... More
A new fluorescence method using the dye FM1-43 was used to examine exocytotic release from hippocampal synaptosomes. Nitric oxide caused a marked transient stimulation of vesicle release. Several structurally unrelated nitric oxide donors, sodium nitroprusside, S-nitroso-N-acetylpenicillamine, 3-morpholino-sydnonimine, and acidified sodium nitrite, were effective. Release stimulated by nitric oxide and KCl ... More
Cytoskeletal breakdown and apoptosis elicited by NO donors in cerebellar granule cells require NMDA receptor activation.
AuthorsBonfoco E, Leist M, Zhivotovsky B, Orrenius S, Lipton SA, Nicotera P
JournalJ Neurochem
PubMed ID8931482
We have recently demonstrated that nitric oxide (NO) donors can trigger either apoptosis or necrosis of neurons as a function of the intensity of the exposure. Here, we show that the apoptosis induced by the NO donors S-nitrosocysteine (SNOC) or S-nitroso-N-acetyl-penicillamine (SNAP) in cultured cerebellar granule cells (CGCs) depends on ... More
Decreased intraocular pressure induced by nitric oxide donors is correlated to nitrite production in the rabbit eye.
AuthorsBehar-Cohen FF, Goureau O, D'Hermies F, Courtois Y
JournalInvest Ophthalmol Vis Sci
PubMed ID8675417
PURPOSE: To evaluate the effect of intraocular administration of nitric oxide (NO) donors in the rabbit eye on intraocular pressure (IOP), inflammation, and toxicity. METHODS: Intravitreal and intracameral injections of two NO donors, SIN-1 and SNAP, and SIN-1C and BSS were performed. Clinical examination, IOP measurements, protein evaluation in aqueous ... More
Nitric oxide and nitric oxide-generating compounds inhibit hepatocyte protein synthesis.
Hepatocytes are stimulated to produce nitric oxide (NO.) from L-arginine in response to conditioned Kupffer cell medium or a combination of cytokines. Associated with the production of NO.in hepatocytes, there is a profound decrease in total protein synthesis ([3H]leucine incorporation). This report demonstrates that authentic NO.and the NO.-generating compound S-nitroso-N-acetylpenicillamine ... More
Detection of nitric oxide from pig trachea by a fluorescence method.
AuthorsLozinsky EM, Martina LV, Shames AI, Uzlaner N, Masarwa A, Likhtenshtein GI, Meyerstein D, Martin VV, Priel Z
JournalAnal Biochem
PubMed ID15003554
A nitronyl nitroxide radical covalently linked to an organic fluorophore, pyrene, was used to detect nitric oxide (NO) from freshly excited tissues. This approach is based on the phenomenon of the intramolecular fluorescence quenching of the fluorophore fragment by the nitroxide. The pyrene-nitronyl (PN) reacts with NO to yield a ... More
Nitric oxide inhibits 3H-glutamate transport in synaptosomes.
AuthorsPogun S, Dawson V, Kuhar MJ
JournalSynapse
PubMed ID7825120
3H-glutamate (GLU) uptake was measured in hippocampal synaptosomes from rat brain. Addition of sodium nitroprusside (SNP) (Sodium nitroferricyanide), a generator of nitric oxide (NO), produced a time-, temperature-, and dose-dependent inhibition of 3H-GLU uptake. The inhibition was due to changes in both Kd and Vmax of GLU uptake, and it ... More
Induction of nitric oxide synthase mRNA expression. Suppression by exogenous nitric oxide.
AuthorsColasanti M, Persichini T, Menegazzi M, Mariotto S, Giordano E, Caldarera CM, Sogos V, Lauro GM, Suzuki H
JournalJ Biol Chem
PubMed ID7592903
The reactive nitrogen species, nitric oxide (NO), plays an important role in the pathogenesis of neurodegenerative diseases. The suppression of NO production may be fundamental for survival of neurons. Here, we report that pretreatment of human ramified microglial cells with nearly physiological levels of exogenous NO prevents lipopolysaccharide (LPS)/tumor necrosis ... More
Ethanol and excitotoxicity in cultured cortical neurons: differential sensitivity of N-methyl-D-aspartate and sodium nitroprusside toxicity.
AuthorsLustig HS, von Brauchitsch KL, Chan J, Greenberg DA
JournalJ Neurochem
PubMed ID1431900
Neural injury due to ischemia and related insults is thought to involve the action of excitatory amino acids at N-methyl-D-aspartate receptors, which results in the influx of extracellular Ca2+ and the generation of nitric oxide. Because ethanol inhibits physiologic responses to excitatory amino acids, we examined its effect on toxicity ... More
Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma and lipopolysaccharide.
AuthorsHenderson SA, Lee PH, Aeberhard EE, Adams JW, Ignarro LJ, Murphy WJ, Sherman MP
JournalJ Biol Chem
PubMed ID7523382
Since early growth response-1 (Egr-1) is required for macrophage differentiation and nitric oxide (NO) is immunosuppressive, we hypothesized that NO would reduce Egr-1 expression in rat lung macrophages. The inflammatory stimuli interferon-gamma and lipopolysaccharide induced an early, transient increase in Egr-1 mRNA (> 5-fold at 2 h) and a sustained, ... More
C11-BODIPY(581/591), an oxidation-sensitive fluorescent lipid peroxidation probe: (micro)spectroscopic characterization and validation of methodology.
AuthorsDrummen GP, van Liebergen LC, Op den Kamp JA, Post JA
JournalFree Radic Biol Med
PubMed ID12160930
C11-BODIPY(581/591) is a fluorescent radio-probe for indexing lipid peroxidation and antioxidant efficacy in model membrane systems and living cells, with excellent characteristics: (i) emission in the visible range of the electromagnetic spectrum, with good spectral separation of the nonoxidized (595 nm) and oxidized (520 nm) forms; (ii) has a high ... More
Inhibition of low-density lipoprotein oxidation by nitric oxide. Potential role in atherogenesis.
AuthorsHogg N, Kalyanaraman B, Joseph J, Struck A, Parthasarathy S
JournalFEBS Lett
PubMed ID8224243
The effects of nitric oxide (.NO) and nitrovasodilators on the oxidation of low-density lipoprotein (LDL) have been studied. S-Nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SNP) inhibited Cu(2+)- and 2,2'-azobis-2-amidinopropane hydrochloride-dependent oxidation of LDL as monitored by oxygen consumption and the formation of thiobarbituric acid-reactive substances, conjugated dienes, and lipid hydroperoxides. In ... More
Regulation of glial cell line-derived neurotrophic factor release from rat C6 glioblastoma cells.
AuthorsVerity AN, Wyatt TL, Hajos B, Eglen RM, Baecker PA, Johnson RM
JournalJ Neurochem
PubMed ID9453547
We have monitored glial cell line-derived neurotrophic factor (GDNF) secretion from rat C6 glioblastoma cells by ELISA. Representative cytokines, neurotrophins, growth factors, neuropeptides, and pharmacological agents were tested for their ability to modulate GDNF release. Whereas most factors tested had minimal effect, a 24-h treatment with fibroblast growth factor-1, -2, ... More
Involvement of reactive oxygen species in cytokine and growth factor induction of c-fos expression in chondrocytes.
AuthorsLo YY, Cruz TF
JournalJ Biol Chem
PubMed ID7744816
The cytokine tumor necrosis factor alpha (TNF alpha) and the growth factor basic fibroblast growth factor (bFGF) are known to induce early response genes such as c-fos and c-jun in various cell types. Activation of AP-1, a heterodimeric complex of Fos and Jun proteins, is required for matrix metalloproteinase production ... More
Nitric oxide-generating vasodilators inhibit mitogenesis and proliferation of BALB/C 3T3 fibroblasts by a cyclic GMP-independent mechanism.
AuthorsGarg UC, Hassid A
JournalBiochem Biophys Res Commun
PubMed ID1697465
The purpose of this study was to investigate the effects of nitric oxide-generating vasodilators and 8-bromo-cGMP on serum-induced mitogenesis in BALB/c 3T3 fibroblasts that lack soluble guanylate cyclase activity. Two such vasodilators, S-nitroso-N-acetylpenicillamine and isosorbide dinitrate, decreased the incorporation of (3H)thymidine in these cells dose-dependently whereas 8-bromo-cGMP was ineffective at ... More
Nitric oxide protects the skeletal muscle Ca2+ release channel from oxidation induced activation.
AuthorsAghdasi B, Reid MB, Hamilton SL
JournalJ Biol Chem
PubMed ID9325258
Reactive oxygen intermediates and nitric oxide modulate the contractile function of skeletal muscle fibers, possibly via direct interaction with the Ca2+ release channel. Oxidants produce disulfide bonds between subunits of the Ca2+ release channel tetramer, and this is accompanied by an increase in channel activity. The sulfhydryl alkylating agent N-ethylmaleimide ... More
Protective effect of nitric oxide against iron-induced neuronal damage.
AuthorsNara K, Konno D, Uchida J, Kiuchi Y, Oguchi K
JournalJ Neural Transm
PubMed ID10599866
We investigated the effect of nitric oxide (NO) on iron-induced neuronal damage. Incubation of PC12 cells after the addition of FeCl2 induced rapid increases (within 1 hr) in lipid peroxidation and a concentration (0.1-2 mM)-dependent decrease in cell viability at 48 hr, both of which were blocked by deferoxamine and ... More
Inhibition of hepatic gluconeogenesis by nitric oxide: a comparison with endotoxic shock.
AuthorsHorton RA, Ceppi ED, Knowles RG, Titheradge MA
JournalBiochem J
PubMed ID8192661
Isolated hepatocytes incubated in the presence of the NO donors S-nitroso-N-acetylpenicillamine (SNAP) and 3-morpholino-sydnonimine (SIN-1) displayed a time- and dose-dependent inhibition of glucose synthesis from lactate plus pyruvate as the substrate which correlated with NO production, but not nitrite production. Neither the parent compound of SNAP, N-acetyl-DL-penicillamine (NAP), nor nitrite ... More
Production of nitric oxide and superoxide by activated macrophages and killing of Leishmania major.
AuthorsAssreuy J, Cunha FQ, Epperlein M, Noronha-Dutra A, O'Donnell CA, Liew FY, Moncada S
JournalEur J Immunol
PubMed ID8125136
Murine macrophages can be activated to produce nitric oxide (NO) and superoxide and these two radicals can react to form peroxynitrite, a powerful oxidant which may be involved in parasite killing. We now show that murine macrophages activated with zymosan and interferon-gamma (ZYM/IFN-gamma) produced both superoxide (peaking 1-2 h after ... More
Nitric oxide modulates low-Mg2+-induced epileptiform activity in rat hippocampal-entorhinal cortex slices.
AuthorsSchuchmann S, Albrecht D, Heinemann U, von Bohlen und Halbach O
JournalNeurobiol Dis
PubMed ID12460549
The production of nitric oxide (NO) during low-Mg2+-induced epileptiform activity in rat hippocampal-entorhinal cortex slices was investigated by real-time monitoring using 1,2-diaminoanthraquinone (DAQ). NO reacts with the aromatic amino groups of DAQ at neutral pH and in the presence of oxygen to form the fluorescence product 1H-anthra-[1,2d]-[1,2,3]triazole-6,11-dione (ATD). The DAQ-induced ... More
Adenosine A3 receptors regulate serotonin transport via nitric oxide and cGMP.
AuthorsMiller KJ, Hoffman BJ
JournalJ Biol Chem
PubMed ID7525554
Many antidepressants inhibit 5-hydroxytryptamine (5HT) transport resulting in increased 5HT levels in the synapse. However, physiological regulation of neurotransmitter uptake has not been demonstrated. We have examined the effect of receptor-activated second messengers on the 5HT transporter in rat basophilic leukemia cells (RBL 2H3). Here, we show that activation of ... More
Differential modulation of prostaglandin H synthase-2 by nitric oxide-related species in intact cells.
Nitrogen monoxide (NO) has been reported to both activate and inhibit prostaglandin (PG) biosynthesis. This apparent paradox might be explained by the production/action of distinct NO-related species formed as a result of the prevailing redox states of different cellular systems. As such, the effect of NO donors with different redox ... More
Inhibition of NF-kappaB and HIV-1 long terminal repeat transcriptional activation by inducible nitric oxide synthase 2 activity.
AuthorsSekkaï D, Aillet F, Israël N, Lepoivre M
JournalJ Biol Chem
PubMed ID9461573
In the human lymphoblastoid T cell line JJhan-5.1, stably transfected with a human immunodeficiency virus-1 long terminal repeat luciferase vector, the level of luciferase activity is dependent on activation of the nuclear factor kappaB (NF-kappaB) transcription factor. Tumor necrosis factor-induced luciferase activity was not modified in JJhan-5.1 cells co-cultivated with ... More
Mechanism of nitric oxide release from S-nitrosothiols.
AuthorsSingh RJ, Hogg N, Joseph J, Kalyanaraman B
JournalJ Biol Chem
PubMed ID8702510
S-Nitrosothiols have many biological activities and have been suggested to be intermediates in signal transduction. The mechanism and products of S-nitrosothiol decomposition are of great significance to the understanding of nitric oxide (.NO) biochemistry. S-Nitrosothiols are stable compounds at 37 degrees C and pH 7.4 in the presence of transition ... More
Stretch activates nitric oxide production in pulmonary vascular endothelial cells in situ.
AuthorsKuebler WM, Uhlig U, Goldmann T, Schael G, Kerem A, Exner K, Martin C, Vollmer E, Uhlig S
JournalAm J Respir Crit Care Med
PubMed ID12947026
Whereas endothelial responses to shear stress have been studied extensively, the responses to circumferential vascular stretch are yet poorly defined. Circumferential stretch in pulmonary microvessels is largely determined by the transmural pressure gradient, hence by both vascular perfusion and alveolar ventilation pressures. Here, we have studied the production of nitric ... More
Effects of the nitric oxide-donor, GEA 3175, on guinea-pig airways.
AuthorsJohansson Rydberg IG, Andersson RG, Grenegård M
JournalEur J Pharmacol
PubMed ID9226411
This investigation characterized the smooth muscle relaxing effect of a novel nitric oxide (NO)-releasing substance, GEA 3175 (1,2,3,4-oxatriazolium, 3-(3-chloro-2-methylphenyl)-5-[[(4-methylphenyl)sulfonyl]amino], hydroxide inner salt) on guinea-pig trachea. GEA 3175 caused a concentration-dependent relaxation of tracheal smooth muscle precontracted with acetylcholine. This effect was reversed by both okadaic acid, an inhibitor of serine/threonine-specific ... More
Reactive oxygen species mediate cytokine activation of c-Jun NH2-terminal kinases.
AuthorsLo YY, Wong JM, Cruz TF
JournalJ Biol Chem
PubMed ID8663189
Interleukin 1 (IL-1) and tumor necrosis factor alpha (TNFalpha) are known to induce production of reactive oxygen species (ROS), which have been suggested to act as second messengers. Here we demonstrate that ROS production by bovine chondrocytes upon cytokine stimulation induces c-jun expression. Since c-jun expression is regulated by its ... More
Nitric oxide inhibits angiotensin II-induced activation of the calcium-sensitive tyrosine kinase proline-rich tyrosine kinase 2 without affecting epidermal growth factor receptor transactivation.
AuthorsWang D, Yu X, Brecher P
JournalJ Biol Chem
PubMed ID10446212
In a previous study, we showed that nitric oxide donors and N-acetylcysteine, either alone or in combination, inhibited the activation of several mitogen-activated protein kinases by angiotensin II in rat cardiac fibroblasts (Wang, D., Yu, X., and Brecher, P. (1998) J. Biol. Chem. 273, 33027-33034). In the present study, we ... More
Induction of nitric oxide synthase in mouse dendritic cells by IFN-gamma, endotoxin, and interaction with allogeneic T cells: nitric oxide production is associated with dendritic cell apoptosis.
AuthorsLu L, Bonham CA, Chambers FG, Watkins SC, Hoffman RA, Simmons RL, Thomson AW
JournalJ Immunol
PubMed ID8871658
Nitric oxide (NO) is an important effector molecule that is involved in immune regulation and host defense. In this study, highly purified NLDC 145+ (DEC-205+) MHC class II(bright) B7-2+ dendritic cells (DC) propagated from normal mouse bone marrow in response to granulocyte-macrophage CSF + IL-4 were induced to produce NO ... More
Modulation of white adipose tissue lipolysis by nitric oxide.
AuthorsGaudiot N, Jaubert AM, Charbonnier E, Sabourault D, Lacasa D, Giudicelli Y, Ribière C
JournalJ Biol Chem
PubMed ID9593681
In isolated adipocytes, the nitrosothiols S-nitroso-N-acetyl-penicillamine (SNAP) and S-nitrosoglutathione stimulate basal lipolysis, whereas the nitric oxide (NO.) donor 1-propamine, 3-(2-hydroxy-2-nitroso-1-propylhydrazine) (PAPA-NONOate) or NO gas have no effect. The increase in basal lipolysis due to nitrosothiols was prevented by dithiothreitol but not by a guanylate cyclase inhibitor. In addition the cyclic ... More
Rebound from nitric oxide inhibition triggers enhanced monocyte activation and chemotaxis.
AuthorsMagazine HI, Chang J, Goumon Y, Stefano GB
JournalJ Immunol
PubMed ID10861041
Exposure of human peripheral blood monocytes to the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP) resulted in a rapid shift in cellular conformation of spontaneously activated cells from ameboid to round. The population of activated cells, approximately 7. 1 +/- 1.2%, was reduced 7-fold to 1.1 +/- 0.4% following 0.5 h exposure to ... More
Nitric oxide inhibits the tumor necrosis factor alpha -regulated endocytosis of human dendritic cells in a cyclic GMP-dependent way.
AuthorsPaolucci C, Rovere P, De Nadai C, Manfredi AA, Clementi E
JournalJ Biol Chem
PubMed ID10777484
Tumor necrosis factor-alpha (TNFalpha)-induced maturation of dendritic cells (DC), with down-regulation of their endocytic ability, has been reported to be mediated by the accumulation of the lipid messenger ceramide. We have now studied the effects and mechanisms of action of NO on endocytosis, investigated with fluorescein isothiocyanate-labeled dextran using human ... More
Nitric oxide induces oxidative stress and apoptosis in neuronal cells.
AuthorsWei T, Chen C, Hou J, Xin W, Mori A
JournalBiochim Biophys Acta
PubMed ID11042352
Within the central nervous system and under normal conditions, nitric oxide (NO) is an important physiological signaling molecule. When produced in large excess, NO also displays neurotoxicity. In our previous report, we have demonstrated that the exposure of neuronal cells to NO donors induced apoptotic cell death, while pretreatment with ... More