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GeneAmp® Thermostable rTth Reverse Transcriptase RNA PCR Kit - FAQs

View additional product information for GeneAmp® Thermostable rTth Reverse Transcriptase RNA PCR Kit - FAQs (N8080069)

2 product FAQs found

Does rTth DNA polymerase (Cat. No. N808-0098) have RNase H activity?

No, rTth DNA polymerase does not have RNase H activity.

Can I use elevated temperatures for reverse transcription with Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase?

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is not well-suited for elevated temperatures. Typically, M-MLV RT operates optimally at temperatures around 37 degrees C to 42 degrees C.

To perform reverse transcription at elevated temperatures (e.g., 50 degrees C or higher), consider using thermostable reverse transcriptases such as SuperScript IV or Maxima H Minus, which are engineered to perform efficiently at higher temperatures.