GeneAmp™ RNA PCR Core Kit

Catalog number: N8080143

Applied Biosystems™  Related applications: Reverse Transcription

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The Applied Biosystems® GeneAmp® RNA PCR Core Kit contains all components necessary to reverse transcribe RNA to cDNA using MuLV(recombinant Moloney murineleukemia virus) reverse transcriptase and to subsequently amplify the synthesized cDNA using the PCR process.

• RNA reverse transcription and cDNA amplification combined in a single tube provides exceptional sensitivity
• The kit protocol enables users to perform a variety of studies including: amplification of cDNA derived from all types of RNA sequences,analysis of gene expression at the RNA level, and detection of specific RNA species present at very low copy number
• The design of the kit eliminates the need to perform large-scale RNA preparations and northern blots

Easy to Use

Both the reverse transcription and amplification reactions take place in a single tube with buffers and reagents co-optimized for these sequential reactions.


See user's manual or package insert for limited label license and trademark information.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 2-Step RT-PCR
Reverse Transcriptase: M-MLV
Polymerase: AmpliTaq® DNA Polymerase
Hot Start: No
Sample Type (General): RNA
Detection Method: Gel Electrophoresis
Final Product Size(s): 5 kb or less
For Use With (Equipment): GeneAmp™ 9700
Primer-Probe Compatibility: Unlabeled Oligos
Product Line: GeneAmp®
Product Size: 1 kit
Shipping Condition: Dry Ice

Contents & storage

Contains MuLV Reverse Transcriptase, AmpliTaq® DNA Polymerase, RNase Inhibitor, dNTPs, GeneAmp® 10X PCR Buffer, MgCl2 Solution, Oligo d(T)16, and Random Hexamers. Sufficient for 100 reverse transcription reactions, 20 µl each and 100 PCR amplifications, 100 µl each. Store at –20°C.


Manuals & protocols

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