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Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer II

Catalog number: N8080161
Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer II

Catalog number: N8080161
Catalog Number
N8080161
Unit Size
250 units
Price (USD)
Price: 122.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5,000 units
Price (USD)
Price: 1,854.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1000 units
Price (USD)
Price: 490.00
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Availability
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Quantity
Catalog Number
Unit Size
25,000 units
Price (USD)
Price: 6,220.00
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Availability
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Quantity
Catalog Number
Unit Size
3,000 units
Price (USD)
Price: 1,196.00
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Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
N8080161250 units
Price: 122.00
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N80801565,000 units
Price: 1,854.00
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***
N80801721000 units
Price: 490.00
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***
N808018625,000 units
Price: 6,220.00
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***
N80801533,000 units
Price: 1,196.00
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***
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Recommendations
Recommendations
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:

  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it good for PCR applications
  • It is QC-tested to guarantee reproducible results

  • Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    For Research Use Only. Not for use in diagnostic procedures.

    Specifications

    For Use With (Application)
    PCR Amplification
    Concentration
    5 U/μL, 10X
    Detection Method
    Primer-Probe
    Fidelity (vs. Taq)
    1 X
    Format
    Stand-alone enzyme
    Hot Start
    No
    No. of Reactions
    200
    Overhang
    3'-A
    Polymerase
    AmpliTaq™ DNA Polymerase
    Reaction Format
    Separate Components
    Reaction Speed
    Standard
    Size (Final Product)
    5 kb or less
    Starting Material
    DNA
    Volume
    1.5 L
    Exonuclease Activity
    5' - 3'
    Product Line
    AmpliTaq™
    Product Type
    DNA Polymerase
    Quantity
    250 Units
    Shipping Condition
    Dry Ice

    Contents & Storage

    Contents:
    • 50 μL of AmpliTaq™ DNA Polymerase (5 U/μL)
    • 1.5 mL of GeneAmp™ 10X PCR Buffer II (100 mM Tris-HCl pH 8.3, 500 mM KCl)
    • 1.5 mL 25 mM Magnesium Chloride
    Store at -20°C.

    Figures