AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
Citations et références (1798)
Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

L’ADN polymérase AmpliTaq est une ADN polymérase recombinante thermostable de 94 kDa obtenue par expression d’une forme modifiée du gèneAfficher plus
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RéférenceComprendQuantité
N8080186Buffer II25,000 unités
N8080161Buffer II250 unités
N8080172Buffer II1000 unités
N8080153Buffer II3000 unités
N8080156Buffer II5 000 unités
N8080152Buffer I3000 unités
N8080155Buffer I5 000 unités
N8080160Buffer I250 unités
N8080171Buffer I1 000 unités
N8080185Buffer I25 000 unités
Référence N8080186
Prix (EUR)
11 437,65
Offre exceptionnelle en ligne
15 050,00
Économisez 3 612,35 (24%)
Each
Ajouter au panier
Comprend:
Buffer II
Quantité:
25,000 unités
Grand volume ou format personnalisé
Prix (EUR)
11 437,65
Offre exceptionnelle en ligne
15 050,00
Économisez 3 612,35 (24%)
Each
Ajouter au panier
L’ADN polymérase AmpliTaq est une ADN polymérase recombinante thermostable de 94 kDa obtenue par expression d’une forme modifiée du gène de l’ADN polymérase Thermus aquaticus (Taq) dans E. coli. Il s’agit de l’enzyme la plus caractérisée et la plus complète disponible pour la PCR. Sa nature recombinante et sa méthode de purification offrent une pureté et une reproductibilité inégalées, flacon à flacon, lot à lot.

Fonctions de cette enzyme :

  • L’ADN polymérase AmpliTaq est l’enzyme la plus caractérisée disponible pour le processus de PCR, ce qui témoigne de son utilité et de son efficacité globales
  • Son profil d’activité thermique la rend idéale pour les applications PCR
  • Son contrôle de qualité est testé pour garantir des résultats reproductibles

    • PCR fiable et robuste
    Le profil d’activité thermique de l’ADN polymérase AmpliTaq est fiable pour les applications de PCR car son activité optimale se situe dans la même plage à laquelle se produit une hybridation rigoureuse des amorces (55 à 75°C). La demi-vie de l’enzyme est d’environ 40 minutes à 95°C, ce qui assure une thermostabilité qui répond aux exigences des applications PCR les plus difficiles. Cette ADN polymérase AmpliTaq est fournie avec le tampon PCR II GeneAmp 10X et la solution MgCl2.

    Pour des performances PCR supérieures, nous recommandons l’ADN polymérase DreamTaq.
    Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
    Spécifications
    Activité exonucléasique5’ à 3’
    Fidélité (par rapport à Taq)1 X
    FormatEnzyme autonome
    Hot StartNon
    ComprendBuffer II
    Nbre de réactions20 000 réactions
    En surplomb3’-A
    PolyméraseADN polymérase AmpliTaq
    Type de produitADN polymérase
    Quantité25,000 unités
    Format de réactionComposants séparés
    Conditions d’expéditionDry Ice
    Taille (produit final)5 kb ou moins
    Matériau de départADN
    Concentration5 U/μl, 10X
    À utiliser avec (application)Standard PCR
    Vitesse de réactionÉtalon
    Unit SizeEach
    Contenu et stockage
    Contenu :
    • 25 x 200 μl d’ADN polymérase AmpliTaq™ (5 U/μl)
    • 100 x 1,5 ml de tampon II de PCR GeneAmp™ 10X (100 mM de Tris-HCl pH 8,3, 500 mM de KCl)
    • 100 x 1,5 ml de chlorure de magnésium à 25 mM
    Stockage à -20°C.

    Foire aux questions (FAQ)

    My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

    Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

    The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

    Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

    I don't see a pellet in my oligo tube order. Should I ask for a replacement?

    The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

    There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

    If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

    There is a green color in my lyophilized oligo. Can I still use it?

    If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

    View all AmpliTaq™ DNA Polymerase with Buffer I or Buffer II FAQs

    Citations et références (1798)

    Citations et références
    Abstract
    Chimpanzee Fab fragments and a derived humanized immunoglobulin G1 antibody that efficiently cross-neutralize dengue type 1 and type 2 viruses.
    Authors:Goncalvez AP; Men R; Wernly C; Purcell RH; Lai CJ
    Journal:Journal of Virology
    PubMed ID:
    Passive immunization with monoclonal antibodies from humans or nonhuman primates represents an attractive alternative to vaccines for prevention of illness caused by dengue viruses (DENV) and other flaviviruses, including the West Nile virus. In a previous study, repertoire cloning to recover Fab fragments from bone marrow mRNA of chimpanzees infected ... More
    Molecular genetics of tetrahydrobiopterin (BH4) deficiency in the Maltese population.
    Authors:Farrugia R; Scerri CA; Montalto SA; Parascandolo R; Neville BG; Felice AE
    Journal:Molecular Genetics and Metabolism
    PubMed ID:
    Deficient activity of the Dihydropteridine Reductase enzyme (DHPR; EC 1.5.1.34; OMIM 261630) is due to mutations in the Quinoid Dihydropteridine Reductase gene on 4p15.3 (QDPR; RefSeq NM_000320). It results in defective recycling of tetrahydrobiopterin (BH(4)) and homozygotes have a rare form of atypical Hyperphenylalaninaemia and Phenylketonuria (aPKU). The heterozygote frequency ... More
    A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast.
    Authors:Eisenkolb M; Zenzmaier C; Leitner E; Schneiter R
    Journal:Molecular Biology of the Cell
    PubMed ID:
    Fungal sphingolipids contain ceramide with a very-long-chain fatty acid (C26). To investigate the physiological significance of the C26-substitution on this lipid, we performed a screen for mutants that are synthetically lethal with ELO3. Elo3p is a component of the ER-associated fatty acid elongase and is required for the final elongation ... More
    Procollagen with skipping of alpha 1(I) exon 41 has lower binding affinity for alpha 1(I) C-telopeptide, impaired in vitro fibrillogenesis, and altered fibril morphology.
    Authors:Cabral WA; Fertala A; Green LK; Korkko J; Forlino A; Marini JC
    Journal:The Journal of Biological Chemistry
    PubMed ID:
    Previous in vitro data on type I collagen self-assembly into fibrils suggested that the amino acid 776-796 region of the alpha1(I) chain is crucial for fibril formation because it serves as the recognition site for the telopeptide of a docking collagen monomer. We used a natural collagen mutation with a ... More
    Vibrio fischeri LuxS and AinS: comparative study of two signal synthases.
    Authors:Lupp C; Ruby EG
    Journal:Journal of Bacteriology
    PubMed ID:
    Vibrio fischeri possesses two acyl-homoserine lactone quorum-sensing systems, ain and lux, both of which are involved in the regulation of luminescence gene expression and are required for persistent colonization of the squid host, Euprymna scolopes. We have previously demonstrated that the ain system induces luminescence at cell densities that precede ... More
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