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Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer II

Catalog number:  N8080186

Related applications:

PCR & Real-Time PCR

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Description

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:

  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it good for PCR applications
  • It is QC-tested to guarantee reproducible results

  • Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    For Research Use Only. Not for use in diagnostic procedures.

    Specifications

    Exonuclease Activity: 5' - 3'
    Fidelity (vs. Taq): 1 X
    Hot Start: No
    Overhang: 3'-A
    Polymerase: AmpliTaq® DNA Polymerase
    Product Line: AmpliTaq™
    Quantity: 25,000 units
    Reaction Format: Separate Components
    Reaction Speed: Standard
    Size (Final Product): 5 kb or less

    Contents & storage

    Contents:
    • 25 x 200 µL of AmpliTaq® DNA Polymerase (5 U/µL)
    • 100 x 1.5 mL of GeneAmp® 10X PCR Buffer II (100 mM Tris-HCl pH 8.3, 500 mM KCl)
    • 100 x 1.5 mL 25 mM Magnesium Chloride
    Store at -20°C.