AmpliTaq® DNA Polymerase with Buffer II (25,000 units/tube)

Catalog number: N8080186

Applied Biosystems™  Related applications: PCR

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Description

AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:

  • AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it reliable for PCR applications
  • It is QC-tested to guarantee reproducible results

  • Reliable and robust PCR
    The thermal activity profile of AmpliTaq® DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer II. It is also available with GeneAmp® 10X PCR Buffer I and MgCl2 Solution.
    For Research Use Only. Not for use in diagnostic procedures.

    Specifications

    Polymerase: AmpliTaq® DNA Polymerase
    Hot Start: No
    Fidelity (vs. Taq): 1 X
    Reaction Format: Separate Components
    Product Overhang: 3'-A
    Reaction Speed: Standard
    Final Product Size(s): 5 kb or less
    Product Size: 25,000 units
    Exonuclease Activity: 5' - 3'

    Contents & storage

    Contents:
    • 25 x 200 µL of AmpliTaq® DNA Polymerase (5 U/µL)
    • 100 x 1.5 mL of GeneAmp® 10X PCR Buffer II (100 mM Tris-HCl pH 8.3, 500 mM KCl)
    • 100 x 1.5 mL 25 mM Magnesium Chloride
    Store at -20°C.

    Documents

    Manuals & protocols