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Applied Biosystems™

AmpliTaq Gold™ DNA Polymerase with Buffer I

Catalog number: N8080240
Applied Biosystems™

AmpliTaq Gold™ DNA Polymerase with Buffer I

Catalog number: N8080240
Catalog Number
N8080240
Unit Size
250 units
Price (USD)
279.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1,000 units
Price (USD)
1,048.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
25 x 1,000 units
Price (USD)
17,460.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
12 x 250 units
Price (USD)
2,590.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 1,000 units
Price (USD)
3,915.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
N8080240250 units
279.00
Your Price:
***
N80802461,000 units
1,048.00
Your Price:
***
N808025825 x 1,000 units
17,460.00
Your Price:
***
N808024412 x 250 units
2,590.00
Your Price:
***
N80802485 x 1,000 units
3,915.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation. Features of this enzyme:

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp™ 10X PCR Buffer I containing 15 mM MgCl2

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold™ DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold™ DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold™ 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold™ 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold™ DNA Polymerase, AmpliTaq Gold™ 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
5 U/µL
Detection Method
Primer-Probe
Fidelity (vs. Taq)
1 X
Format
Stand-alone enzyme
GC-Rich PCR Performance
Low
Hot Start
Built-In Hot Start
No. of Reactions
200
Overhang
3'-A
Polymerase
AmpliTaq Gold DNA Polymerase
Reaction Format
Separate Components
Reaction Speed
Standard
Size (Final Product)
5 kb or less
Starting Material
DNA
Exonuclease Activity
5' - 3'
Includes
AmpliTaq Gold DNA Polymerase (250 units) and Buffer I.
Product Line
AmpliTaq Gold™
Product Type
DNA Polymerase With Buffer I
Quantity
250 Units
Shipping Condition
Dry Ice

Contents & Storage

• 1 x 250 U AmpliTaq Gold DNA Polymerase
• 1 x 1.5 mL 10X PCR Buffer I containing MgCl2

Store at –15 to –30°C.

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