What additional analytes or biomolecules are routinely analyzed using the NanoDrop 3300 Fluorospectrometer?
The NanoDrop 3300 is routinely used for the following:
- Determine the concentration of nucleic acids (some of the pre-configured methods are dsDNA Hoechst dye, dsDNA PicoGreen dye and RNA RiboGreen dye)
- Characterize molecular beacon probes labeled with different fluorophores
- Assess the fluorescence of dyes such as Alexa, Cy and DyLightdyes
- Measure fluorescence of a wide variety of inherent fluorophores
Can I simply wipe the pedestal surface, of the NanoDrop 3300 Fluorospectrometer, enough to prevent carryover?
Yes, we recommend using dry laboratory wipes as a very effective method for removing the sample. The highly polished quartz and stainless-steel surfaces of the sample retention system are resistant to sample adherence.
What is the dynamic range for proteins that can be measured on the NanoDrop 3300 Fluorospectrometer?
Using a 2 µL sample, the range using the Quant-iT protein assay is 0.01 µg - 1.0 µg (BSA).
The range using the fluorescein assay is > 4 logs.
Detailed performance data using other fluorophores may be found on the Thermo Fisher website.
Is the NanoDrop 3300 Fluorospectrometer affordable, even though it allows for a large amount of flexibility?
The NanoDrop 3300 Fluorospectrometer is very affordable compared to high-end fluorescence instruments, primarily because the NanoDrop 3300 Fluorospectrometer does not use costly monochromators.
Can I use the NanoDrop 3300 Fluorospectrometer if I do not know what excitation source to use for my sample?
The Fluorescence Profiler feature, in the NanoDrop 3300 Fluorospectrometer, allows LED excitation selection guidance when you are unsure of the excitation source and/or emission profile of a specific or unknown fluorophore.