'Matrix GLA protein (MGP) has been identified as a calcification inhibitor in cartilage and vasculature. Part of this effect may be attributed to its influence on osteoinductive activity of bone morphogenetic protein-2 (BMP-2). To detect binding between MGP and BMP-2, we performed immunoprecipitation using MGP and BMP-2 tagged with FLAG ... More
Differentiation of mouse embryonic stem cells following RNAi-mediated silencing of OCT4 and Nanog.
AuthorsHough SR, Clements I, Welch PJ, Wiederholt KA,
JournalStem Cells
PubMed ID16456133
RNAi holds great promise as a tool to study the basic biology of stem cells or to direct differentiation in a specific manner. Barriers to achieving efficient and specific gene silencing in RNAi experiments include limitations in transfection efficiency and in the efficacy and specificity of RNAi silencing effectors. Here, ... More
Oligomerization of the fifth transmembrane domain from the adenosine A2A receptor.
AuthorsThévenin D, Lazarova T, Roberts MF, Robinson CR,
JournalProtein Sci
PubMed ID15987888
The human adenosine A2A receptor (A(2A)R) belongs to one of the largest family of membrane proteins, the G-protein coupled receptors (GPCRs), characterized by seven transmembrane (TM) helices. Little is known about the determinants of their structures, folding, assembly, activation mechanisms, and oligomeric states. Previous studies in our group showed that ... More
A regulatory light chain of ciliary outer arm dynein in Tetrahymena thermophila.
AuthorsChristensen ST, Guerra C, Wada Y, Valentin T, Angeletti RH, Satir P, Hamasaki T,
JournalJ Biol Chem
PubMed ID11274140
Ciliary beat frequency is primarily regulated by outer arm dyneins (22 S dynein). Chilcote and Johnson (Chilcote, T. J., and Johnson, K. A. (1990) J. Biol. Chem. 256, 17257-17266) previously studied isolated Tetrahymena 22 S dynein, identifying a protein p34, which showed cAMP-dependent phosphorylation. Here, we characterize the molecular biochemistry ... More
Identification and expression of immunogenic proteins of a disease-associated marine turtle herpesvirus.
AuthorsCoberley SS, Condit RC, Herbst LH, Klein PA,
JournalJ Virol
PubMed ID12239336
Herpesviruses are associated with several diseases of marine turtles, including lung-eye-trachea disease (LETD) and fibropapillomatosis. Two approaches were used to identify immunodominant antigens of LETV, the LETD-associated herpesvirus. The first approach targeted glycoprotein B, which is known to be immunogenic and neutralizing in other species. The second strategy identified LETV ... More
Rapid exchange of actin-bound nucleotide in perfused rat heart.
AuthorsBárány M, de Tombe PP,
JournalAm J Physiol Heart Circ Physiol
PubMed ID15020303
In the rat heart the actin-bound nucleotide contained both ATP and ADP. The ratio of bound ATP to bound ADP depended on the functional state of the heart; it was higher in hearts stopped reversibly in diastole (low Ca(2+), high Mg(2+), or high K(+)), than in stimulated (inotropic agents or ... More