NuPAGE™ Bis-Tris Resupply Pack, 4 to 12%

The NuPAGE Bis-Tris Resupply Pack contains:

• NuPAGE Bis-Tris gels (10 boxes, 100 gels)
• NuPAGE MES Running Buffer for 100 gel runs, 20X (4 bottles, Cat. No. NP0002)
• PageRuler Plus Pre-stained Protein Ladder for 100 gel runs, 10 to 250 kDa (Cat. No. 26619)

About NuPAGE Gels

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed for optimal separation of a wide range of proteins under denaturing conditions. Their neutral pH environment is key to minimizing protein modifications, making them ideal for applications where maintaining protein integrity is crucial, such as sequencing and mass spectrometry. These gels offer fast protein separation, delivering results in as little as 35 minutes. Additionally, their long shelf-life of 16 months at room temperature adds to their practicality and convenience, making them an excellent choice for everyday laboratory use.

Separation

For running Invitrogen protein gels, you have the flexibility to use either the Mini Gel Tank (A25977) or the XCell SureLock Mini-Cell (EI0001). Both systems are designed to provide optimal performance. The Mini Gel Tank's unique side-by-side design offers efficient and convenient electrophoresis for both precast and SureCast handcast mini gels. Alternatively, the XCell SureLock Mini-Cell allows quick, easy, and leak-free running of mini gels, without the need for any clamps. This compatibility gives you the freedom to choose the best tool for your specific lab and experiment requirements. Additionally, the Mini Gel Tank is conveniently available through Invitrogen Precast Gel Welcome Packs along with both sample buffer and reducing agent, providing an all-in-one solution for your electrophoresis needs.

Transfer

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer Buffer (NP0006) for traditional wet transfer using the Mini Blot Module (B1000). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).