Invitrogen™

Bolt™ 8%, Bis-Tris, 1.0 mm, Mini Protein Gels

Catalog number: NW00087BOX
Invitrogen™

Bolt™ 8%, Bis-Tris, 1.0 mm, Mini Protein Gels

Catalog number: NW00087BOX
Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial.
 
Catalog Number
NW00087BOX
Unit Size
10 gels (1 box)
Wells
17-well
Price (USD)
157.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 30 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life16 Months
Wells17-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
10-well
Price (USD)
157.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 60 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life16 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
12-well
Price (USD)
157.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 45 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life16 Months
Wells12-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
15-well
Price (USD)
157.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 35 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life16 Months
Wells15-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeWellsPrice (USD)AvailabilityQuantity
NW00087BOXFull specifications
10 gels (1 box)17-well
157.00
Your Price:
***
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 30 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life16 Months
Wells17-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NW00080BOXFull specifications
10 gels (1 box)10-well
157.00
Your Price:
***
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 60 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life16 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NW00082BOXFull specifications
10 gels (1 box)12-well
157.00
Your Price:
***
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 45 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life16 Months
Wells12-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NW00085BOXFull specifications
10 gels (1 box)15-well
157.00
Your Price:
***
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage8%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 35 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life16 Months
Wells15-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Showing 4 of 4

Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank (A25977) is required for use of all Bolt gels.

Features include:
Optimized run conditions—separate your proteins using constant voltage in approximately 20 minutes
Long shelf life—store gels for at least 16 months at 4–25°C
Better protein integrity—neutral pH sample preparation process preserves your proteins
Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS (B000202) or Bolt MOPS SDS (B000102) running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 20 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer (BT0006) for traditional wet transfer using the Mini Blot Module (B1000) . Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the Blot 2 Gel Transfer Device (IB21001).

For Research Use Only. Not for use in diagnostic procedures.

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