This product is designed for use with the new Mini Gel Tank ( A25977) or by retrofitting the Bolt Mini Gel Tank ( B4477599) with the Cassette Clamp Cam Handles Set ( A26732).

Bolt™ 10% Bis-Tris Plus Gels, 10-well

Catalog number: NW00100BOX

Invitrogen™  Related applications: Protein Gel Electrophoresis

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Description

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank is required for use of all Bolt gels.

Features include:
• Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
• Long shelf life—store gels for at least 16 months at 4–25°C
• Better protein integrity—neutral pH sample preparation process preserves your proteins
• Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Learn more about all of our available Bolt Bis-Tris Plus gels >

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using Bolt Transfer buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Mini Blot Module.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Percentage: 10 %
Wells: 10 wells
Gel Type: Bis-Tris Plus Gel
Separation Range: 15 kDa to 260 kDa (MOPS Buffer), 3.5 kDa to 160 kDa (MES Buffer)
Gel Size: Mini (8 cm x 8 cm)
Well Format: 1D Well (Wedge-Shaped)
Thickness: 1.0 mm
Product Line: Bolt™
Adapters: No Adapters
Capacity: Up to 60 µL per well
For Use With (Equipment): Mini Gel Tank
Mode of Separation: Molecular Weight
Product Size: 10 gels (1 box)
Quantity: 10 gels
Sample Loading Volume: 40 µL (recommended)
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

One box contains 10 gels. Store at 4–25°C. Shelf life is 16 months.

Documents

Manuals & protocols

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