The new Mini Gel Tank includes cassette clamps with gray cam handles designed for use with the new 10 cm Bolt® mini gels as well as existing NuPAGE® and Novex® Tris-Glycine mini gels.

Bolt™ Welcome Pack + iBlot™ 2 System

Catalog number:  NW0412AIB2

 Related applications:

Protein Electrophoresis & Western Blotting

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The Bolt® Welcome Pack + iBlot® 2 System offers a complete protein separation-to-western blot solution by combining a Bolt® Welcome Pack A with the iBlot® 2 Gel Transfer Device and iBlot® 2 Transfer Stack (nitrocellulose, regular size) in lieu of one box of Bolt® gels.

The Bolt® Welcome Pack A consists of a Mini Gel Tank plus all of the necessary Bolt® gels and buffers you will need to get started running gels.

Contents of the Bolt® Welcome Pack A:
Mini Gel Tank
Bolt® 4-12% Bis-Tris Plus gels, 10-well (1 box)
Bolt® MES Running Buffer (20X)
Bolt® LDS Sample Buffer (4X)
Bolt® Sample Reducing Agent (10X)
SeeBlue® Plus2 Pre-Stained Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with a variety of Novex® gels, NuPAGE® gels, and Bolt® Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt® Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used.

About Bolt® Bis-Tris Plus Gels
Bolt® Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE® Bis-Tris gels, Bolt® Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt® gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt® gels to obtain optimal results for your day-to-day protein separation needs. Bolt® Bis-Tris Plus gels come in four g types and multiple well formats. Each box contains 10 gels.

About the iBlot® 2 Gel Transfer Device
The iBlot® 2 Gel Transfer Device is our second-generation dry-transfer device, providing the same speed and convenience as the original iBlot® device, but with many new features. The iBlot® 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes or less, and without the need for liquid buffers. The iBlot® 2 Gel Transfer Device is an integral part of the iBlot® 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).
WARNING: Cancer and Reproductive Harm –
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Bolt®, iBlot™
Capacity: Up to 2 gels per run
Gel Compatibility: Novex® Mini Gels
Product Line: Bolt™
Product Size: 1 kit
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

• Mini Gel Tank
• 1 box of Bolt™ 4-12% Bis-Tris Plus gels, 10-well (10 gels)
• 500 mL of Bolt™ MES SDS Running Buffer (20X)
• 10 mL of Bolt™ LDS Sample Buffer (4X)
• 10 mL of Bolt™ Sample Reducing Agent (10X)
• 500 µL SeeBlue™ Plus2 Pre-Stained Standard
• iBlot™ 2 Gel Transfer Device
• one package of iBlot™ 2 Transfer Stack Regular Nitrocelluose (10 stacks)


Manuals & protocols