The new Mini Gel Tank includes cassette clamps with gray cam handles designed for use with the new 10 cm Bolt® mini gels as well as existing NuPAGE® and Novex® Tris-Glycine mini gels.

Bolt™ Welcome Pack + iBlot™ 2 System

Catalog number: NW0412AIB2


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The Bolt® Welcome Pack + iBlot® 2 System offers a complete protein separation-to-western blot solution by combining a Bolt® Welcome Pack A with the iBlot® 2 Gel Transfer Device and iBlot® 2 Transfer Stack (nitrocellulose, regular size) in lieu of one box of Bolt® gels.

The Bolt® Welcome Pack A consists of a Mini Gel Tank plus all of the necessary Bolt® gels and buffers you will need to get started running gels.

Contents of the Bolt® Welcome Pack A:
Mini Gel Tank
Bolt® 4-12% Bis-Tris Plus gels, 10-well (1 box)
Bolt® MES Running Buffer (20X)
Bolt® LDS Sample Buffer (4X)
Bolt® Sample Reducing Agent (10X)
SeeBlue® Plus2 Pre-Stained Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with a variety of Novex® gels, NuPAGE® gels, and Bolt® Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt® Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used.

About Bolt® Bis-Tris Plus Gels
Bolt® Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE® Bis-Tris gels, Bolt® Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt® gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt® gels to obtain optimal results for your day-to-day protein separation needs. Bolt® Bis-Tris Plus gels come in four g types and multiple well formats. Each box contains 10 gels.

About the iBlot® 2 Gel Transfer Device
The iBlot® 2 Gel Transfer Device is our second-generation dry-transfer device, providing the same speed and convenience as the original iBlot® device, but with many new features. The iBlot® 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes or less, and without the need for liquid buffers. The iBlot® 2 Gel Transfer Device is an integral part of the iBlot® 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): iBlot™, Bolt®
Capacity: Up to 2 gels per run
Product Size: 1 kit
Gel Compatibility: Novex® Mini Gels
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

• Mini Gel Tank
• 1 box of Bolt™ 4-12% Bis-Tris Plus gels, 10-well (10 gels)
• 500 mL of Bolt™ MES SDS Running Buffer (20X)
• 10 mL of Bolt™ LDS Sample Buffer (4X)
• 10 mL of Bolt™ Sample Reducing Agent (10X)
• 500 µL SeeBlue™ Plus2 Pre-Stained Standard
• iBlot™ 2 Gel Transfer Device
• one package of iBlot™ 2 Transfer Stack Regular Nitrocelluose (10 stacks)


Manuals & protocols

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