Phorbol ester induces elevated oxidative activity and alkalization in a subset of lysosomes.
AuthorsChen CS
JournalBMC Cell Biol
PubMed ID12165102
'BACKGROUND: Lysosomes are acidic organelles that play multiple roles in various cellular oxidative activities such as the oxidative burst during cytotoxic killing. It remains to be determined how lysosomal lumen oxidative activity and pH interact and are regulated. Here, I report the use of fluorescent probes to measure oxidative activity ... More
Time to fatigue is increased in mouse muscle at 37 degrees C; the role of iron and reactive oxygen species.
AuthorsReardon TF, Allen DG,
JournalJ Physiol
PubMed ID19470779
'Studies exploring the rate of fatigue in isolated muscle at 37 degrees C have produced mixed results. In the present study, muscle fibre bundles from the mouse foot were used to study the effect of temperature on the rate of muscle fatigue. Provided iron was excluded from the solutions, time ... More
Nox2 and Rac1 regulate H2O2-dependent recruitment of TRAF6 to endosomal interleukin-1 receptor complexes.
AuthorsLi Q, Harraz MM, Zhou W, Zhang LN, Ding W, Zhang Y, Eggleston T, Yeaman C, Banfi B, Engelhardt JF
JournalMol Cell Biol
PubMed ID16354686
'Reactive oxygen species (ROS) generated by NADPH oxidases (Nox) have been implicated in the regulation of signal transduction. However, the cellular mechanisms that link Nox activation with plasma membrane receptor signaling remain poorly defined. We have found that Nox2-derived ROS influence the formation of an active interleukin-1 (IL-1) receptor complex ... More
An apoplastic h-type thioredoxin is involved in the stress response through regulation of the apoplastic reactive oxygen species in rice.
AuthorsZhang CJ, Zhao BC, Ge WN, Zhang YF, Song Y, Sun DY, Guo Y,
JournalPlant Physiol
PubMed ID22010108
'Thioredoxins (Trxs) are a multigenic family of proteins in plants that play a critical role in redox balance regulation through thiol-disulfide exchange reactions. There are 10 members of the h-type Trxs in rice (Oryza sativa), and none of them has been clearly characterized. Here, we demonstrate that OsTRXh1, a subgroup ... More
Decreased superoxide production in macrophages of long-lived p66Shc knock-out mice.
AuthorsTomilov AA, Bicocca V, Schoenfeld RA, Giorgio M, Migliaccio E, Ramsey JJ, Hagopian K, Pelicci PG, Cortopassi GA,
JournalJ Biol Chem
PubMed ID19892704
'A decrease in reactive oxygen species (ROS) production has been associated with extended life span in animal models of longevity. Mice deficient in the p66Shc gene are long-lived, and their cells are both resistant to oxidative stress and produce less ROS. Our microarray analysis of p66Shc(-/-) mouse tissues showed alterations ... More
Caveosomal oxidative stress causes Src-p21ras activation and lysine 63 TRAF6 protein polyubiquitination in iron-induced M1 hepatic macrophage activation.
AuthorsZhong S, Xu J, Li P, Tsukamoto H,
JournalJ Biol Chem
PubMed ID22829592
'Proinflammatory M1 activation of hepatic macrophages (HM) is critical in pathogenesis of hepatitis, but its mechanisms are still elusive. Our earlier work demonstrates the role of ferrous iron (Fe(2+)) as a pathogen-associated molecular pattern-independent agonist for activation of I?B kinase (IKK) and NF-?B in HM via activation and interaction of ... More
Ca2+ regulates reactive oxygen species production and pH during mechanosensing in Arabidopsis roots.
'Mechanical stimulation of plants triggers a cytoplasmic Ca(2+) increase that is thought to link the touch stimulus to appropriate growth responses. We found that in roots of Arabidopsis thaliana, external and endogenously generated mechanical forces consistently trigger rapid and transient increases in cytosolic Ca(2+) and that the signatures of these ... More
NADPH oxidase and extracellular regulated kinases 1/2 are targets of prion protein signaling in neuronal and nonneuronal cells.
AuthorsSchneider B, Mutel V, Pietri M, Ermonval M, Mouillet-Richard S, Kellermann O
JournalProc Natl Acad Sci U S A
PubMed ID14597699
'Putative functions of the cellular prion protein, PrPC, include resistance to oxidative stress, copper uptake, cell adhesion, and cell signaling. Here, we report NADPH oxidase-dependent reactive oxygen species (ROS) production and extracellular regulated kinases 1/2 (ERK1/2) phosphorylation on PrPC stimulation in the 1C11 neuroectodermal precursor, in its neuronal differentiated progenies, ... More
Alsin and SOD1(G93A) proteins regulate endosomal reactive oxygen species production by glial cells and proinflammatory pathways responsible for neurotoxicity.
AuthorsLi Q, Spencer NY, Pantazis NJ, Engelhardt JF,
JournalJ Biol Chem
PubMed ID21937428
'Recent studies have implicated enhanced Nox2-mediated reactive oxygen species (ROS) by microglia in the pathogenesis of motor neuron death observed in familial amyotrophic lateral sclerosis (ALS). In this context, ALS mutant forms of SOD1 enhance Rac1 activation, leading to increased Nox2-dependent microglial ROS production and neuron cell death in mice. ... More
Neutrophil serine proteases play an important role in inflammation by modulating neutrophil effector functions. We have previously shown that neutrophils deficient in the serine proteases cathepsin G and neutrophil elastase (CG/NE neutrophils) exhibit severe defects in chemokine CXCL2 release and reactive oxygen species (ROS) production when activated on immobilized immune ... More
Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs.
AuthorsMonshausen GB, Bibikova TN, Messerli MA, Shi C, Gilroy S,
JournalProc Natl Acad Sci U S A
PubMed ID18079291
Root hairs show highly localized cell expansion focused to their growing tips. This growth pattern is accomplished through restriction of secretion to the elongating apex and modulation of cell wall properties, with the wall just behind the tip becoming rigidified to resist the lateral expansive forces of turgor. In this ... More
Endotoxin priming of neutrophils requires endocytosis and NADPH oxidase-dependent endosomal reactive oxygen species.
NADPH oxidase 2 (Nox2)-generated reactive oxygen species (ROS) are critical for neutrophil (polymorphonuclear leukocyte (PMN)) microbicidal function. Nox2 also plays a role in intracellular signaling, but the site of oxidase assembly is unknown. It has been proposed to occur on secondary granules. We previously demonstrated that intracellular NADPH oxidase-derived ROS ... More
Autocrine/paracrine pattern of superoxide production through NAD(P)H oxidase in coronary arterial myocytes.
AuthorsZhang G, Zhang F, Muh R, Yi F, Chalupsky K, Cai H, Li PL
JournalAm J Physiol Heart Circ Physiol
PubMed ID16963617
The present study tested the hypothesis that membrane-bound NAD(P)H oxidase in coronary arterial myocytes (CAMs) is capable of producing superoxide (O(2)(*-)) toward extracellular space to exert an autocrine- or paracrine-like action in these cells. Using a high-speed wavelength-switching fluorescent microscopic imaging technique, we simultaneously monitored the binding of dihydroethidium-oxidizing product ... More
Membrane capacitance changes associated with particle uptake during phagocytosis in macrophages.
AuthorsHolevinsky KO, Nelson DJ
JournalBiophys J
PubMed ID9788954
We report the use of capacitance measurements to monitor particle uptake after cellular exposure to phagocytic stimuli. In these studies, human monocyte-derived macrophages (HMDMs) and cells from the murine macrophage-like cell line J774.1 were exposed to immune complexes or sized latex particles (0.8 or 3.2 micron in diameter). An average ... More