Profiling antibody responses by multiparametric analysis of primary B cells.
AuthorsStory CM, Papa E, Hu CC, Ronan JL, Herlihy K, Ploegh HL, Love JC,
JournalProc Natl Acad Sci U S A
PubMed ID19004776
'Determining the efficacy of a vaccine generally relies on measuring neutralizing antibodies in sera. This measure cannot elucidate the mechanisms responsible for the development of immunological memory at the cellular level, however. Quantitative profiles that detail the cellular origin, extent, and diversity of the humoral (antibody-based) immune response would improve ... More
A microengraving method for rapid selection of single cells producing antigen-specific antibodies.
AuthorsLove JC, Ronan JL, Grotenbreg GM, van der Veen AG, Ploegh HL
JournalNat Biotechnol
PubMed ID16699501
Monoclonal antibodies that recognize specific antigens of interest are used as therapeutic agents and as tools for biomedical research. Discovering a single monoclonal antibody requires retrieval of an individual hybridoma from polyclonal mixtures of cells producing antibodies with a variety of specificities. The time required to isolate hybridomas by a ... More
Microscale Symmetrical Electroporator Array as a Versatile Molecular Delivery System.
Authors
JournalSci Rep
PubMed ID28317836
Conserved meningeal lymphatic drainage circuits in mice and humans.
Authors
JournalJ Exp Med
PubMed ID35776089
Attenuation of cerebral edema facilitates recovery of glymphatic system function after status epilepticus.
Authors
JournalJCI Insight
PubMed ID34494549
Anatomy and function of the vertebral column lymphatic network in mice.
Authors
JournalNat Commun
PubMed ID31597914
A sequential 3D bioprinting and orthogonal bioconjugation approach for precision tissue engineering.