(I-pyrenebutyryl)carnitine and 1-pyrenebutyryl coenzyme A: fluorescent probes for lipid metabolite studies in artificial and natural membranes.
AuthorsWolkowicz PE, Pownall HJ, McMillin-Wood JB
JournalBiochemistry
PubMed ID7104308
'Membrane properties of fatty acyl coenzyme A (CoA) and acylcarnitine have been studied with 1-pyrenebutyryl-CoA and (1-pyrenebutyryl) carnitine. These molecules have a spectroscopic properties of pyrene and its derivatives and exhibit biological and chemical characteristics related to acyl esters. (1-Pyrenebutyryl) carnitine (PBC) is more soluble in nonpolar solvents than 1-pyrenebutyryl-CoA ... More
Enhanced association of platelet-activating factor acetylhydrolase with lipoprotein (a) in comparison with low density lipoprotein.
AuthorsBlencowe C, Hermetter A, Kostner GM, Deigner HP
JournalJ Biol Chem
PubMed ID8537378
'Paired samples of human Lipoprotein (a) (Lp(a)) and low density lipoprotein (LDL) were assayed for their platelet-activating factor (PAF) acetylhydrolase activity. Lp(a) displayed markedly enhanced PAF acetylhydrolase activity (approximately 7-fold based on equal particle concentrations) in comparison to LDL isolated from the same individual. Lp(a)-associated acetylhydrolase exhibited properties observed for ... More
Fluorescent phosphonate label for serine hydrolases, pyrenebutyl methylphosphonofluoridate: reaction with acetylcholinesterase.
AuthorsBerman HA, Taylor P
JournalBiochemistry
PubMed ID566114
Ultraviolet and fluorescence derivatization reagents for carboxylic acids suitable for high performance liquid chromatography: a review.
AuthorsMukherjee PS, Karnes HT
JournalBiomed Chromatogr
PubMed ID8879524
Pre- and postcolumn derivatization with a suitable chromophore or fluorophore have often been utilized to enhance the sensitivity and selectivity of detection for analytes possessing a carboxyl group. The major classes of UV and fluorescent derivatization reagents include the coumarin analogues, alkyl halides, diazoalkanes, and amines. The derivatization reaction conditions, ... More
Allosteric mechanisms in P450eryF probed with 1-pyrenebutanol, a novel fluorescent substrate.
AuthorsDavydov DR, Kumar S, Halpert JR
JournalBiochem Biophys Res Commun
PubMed ID12061778
1-Pyrenebutanol (1-PB) has been used as a new fluorescent substrate for P450eryF to explore the molecular mechanisms of cooperativity. Hydroxylation of 1-PB by P450eryF was detected by both fluorometric and chromatographic assays. Binding was monitored by a substrate-induced low-to-high spin shift, as well as by fluorescence resonance energy transfer (FRET) ... More
Interaction of S-acyl fatty acid synthase thioester hydrolase with fatty acid synthase. Direct measurement of binding by fluorescence anisotropy.
AuthorsFoster RJ, Bonsall RF, Poulose AJ, Kolattukudy PE
JournalJ Biol Chem
PubMed ID3968077
Treatment of S-acyl fatty acid synthase thioester hydrolase from the uropygial gland of Peking duck with pyrenebutylmethanephosphonofluoridate resulted in inactivation of the enzyme with covalent attachment of the pyrene derivative to the enzyme. One mole of the derivative was attached/mol of protein, most probably at the active serine. When avian ... More
Aged and non-aged pyrenebutyl-containing organophosphoryl conjugates of chymotrypsin. Preparation and comparison by 31P-NMR spectroscopy.
AuthorsGrunwald J, Segall Y, Shirin E, Waysbort D, Steinberg N, Silman I, Ashani Y
JournalBiochem Pharmacol
PubMed ID2818617
Homologous pairs of non-aged and aged pyrene-containing phosphoryl conjugates of chymotrypsin were prepared in order to characterize by NMR and optical spectroscopy putative differences in the conformation of non-aged and aged organophosphoryl conjugates of serine hydrolases. Pyrenebutyl-O-P(O)(OC2H5)F and pyrenebutyl-O-P(O)(OC2H5)Cl were used to obtain the non-aged form pyrenebutyl-O-P(O)(OC2H5)-Cht, whereas pyrenebutyl-O-P(O)Cl2, pyrenebutyl-O-P(O)(p-nitrophenoxy)Cl, ... More
In situ time-resolved fluorescence spectroscopy in the frequency domain in capillary electrochromatography.
AuthorsHe Y, Geng L
JournalAnal Chem
PubMed ID11985313
In situ time-resolved fluorescence spectroscopy for capillary electrochromatography (CEC) is described in the frequency domain. Fluorescence decay of the solute molecules is collected directly in the packed stationary phase of the CEC capillary. The fluorescence lifetime profile of the solute molecules reveals the microenvironments they experience in the C18 chromatographic ... More
Pyrene butanol--an efficient, selective and non-metabolized photosensitizing agent for human myeloid leukemia cells.
AuthorsFibach E, Rabia SA, Gatt S
JournalLeuk Res
PubMed ID1625470
Methods for ex vivo purging of neoplastic cells from harvested marrow are being developed to increase the efficacy of autologous transplantation. One approach is selective photosensitization, using sensitizing compounds and light radiation. Pyrene-containing fatty acids and lipids are potent photosensitizers, e.g. 12-(1-pyrene)dodecanoic acid (P12), is taken up preferentially by leukemic ... More
Conformational differences between aged and nonaged pyrenebutyl-containing organophosphoryl conjugates of chymotrypsin as detected by optical spectroscopy.
AuthorsSteinberg N, van der Drift AC, Grunwald J, Segall Y, Shirin E, Haas E, Ashani Y, Silman I
JournalBiochemistry
PubMed ID2713362
Homologous aged and nonaged fluorescent organophosphorus conjugates of alpha-chymotrypsin (Cht) were used in a comparative spectroscopic study of the conformation of their active sites, employing the pyrene group as the fluorescent probe. Steady-state fluorescence measurements showed that the quantum yield of the pyrene probe which is stoichiometrically attached to the ... More
Analysis of heterogeneous fluorescence decays. Distribution of pyrene derivatives in an octadecylsilane layer in capillary electrochromatography.
AuthorsHe Y, Geng L
JournalAnal Chem
PubMed ID11816589
The distribution of solute molecules in the stationary phase in capillary electrochromatography (CEC) has been investigated with time-resolved fluorescence in the frequency domain. The analysis of fluorescence decay poses a challenging problem for the complex decay kinetics of heterogeneous systems such as the C18 stationary phase. The nonlinear least-squares (NLLS) ... More
Boronic acid fluorophore/beta-cyclodextrin complex sensors for selective sugar recognition in water.
AuthorsTong AJ, Yamauchi A, Hayashita T, Zhang ZY, Smith BD, Teramae N
JournalAnal Chem
PubMed ID11321305
A novel boronic acid fluorophore 1/beta-cyclodextrin (beta-CyD) complex sensor for sugar recognition in water has been designed. The probe 1 bearing pyrene moiety as a fluorescent signal transducer exhibits no fluorescence emission, due to its aggregation in water containing 2% DMSO; however, the addition of beta-CyD to this solution largely ... More