Vivid® BOMF Substrate

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Vivid® Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product. They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. When used in combination with CYP450 BACULOSOMES® Plus Reagents as part of the Vivid® CYP450 Screening Kits, the Vivid® Substrates enable rapid, robust detection of cytochrome P450 inhibitors. This particular substrate (Vivid ® BOMF Substrate) yields a product that emits a green fluorescence.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Substrate: BOMF
Product Size: 0.1 mg
Substrate Type: Cytochrome P450 Substrate
Detection Method: Fluorescent
Substrate Properties: Chemical Substrate
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Vivid® CYP450 Screening kits include Vivid® CYP450 Fluorogenic substrates, BACULOSOMES® CYP450 reagents, fluorescent standards, and an NADPH-regeneration system. Vivid® CYP450 Fluorogenic substrates are also available separately. Store reaction buffers at +4°C or room temperature. Store BACULOSOMES®, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid® substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)


Manuals & protocols

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