Vivid™ Green Fluorescent Standard

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Vivid® Fluorescent Standards represent the products formed during oxidation of Vivid® Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid® CYP450 Screening Kits. Vivid® Fluorescent Standards are included in the Vivid® Screening Kits, but may also be purchased separately. They are supplied as a dried film.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. Vivid® Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition For Research Use Only. Not for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Product Line: VIVID®
Product Size: 1 each
Shipping Condition: Wet Ice

Contents & storage

Vivid® CYP450 Screening kits include Vivid® CYP450 Fluorogenic substrates, BACULOSOMES® CYP450 reagents, fluorescent standards, and an NADPH-regeneration system. Vivid® CYP450 Fluorogenic substrates are also available separately. Store reaction buffers at +4°C or room temperature. Store BACULOSOMES®, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid® substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)


Manuals & protocols