Vivid™ Regeneration System, 100X, is a Vivid™ CYP450 Screening Kit component consisting of glucose-6-phosphate and glucose-6-phosphate dehydrogenase, which converts NADP+ into NADPH, which is required to start the CYP450 reaction. Vivid™ Regeneration System, 100X, is included in the Vivid™ Screening Kits, but may also be purchased separately.
More About Vivid™ CYP450 Screening Kits Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagent. Vivid™ Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.