Vivid™ DBOMF Substrate
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Thermo Scientific™

Vivid™ DBOMF Substrate

Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either ofRead more
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Catalog NumberQuantity
P29791 mg
P29740.1 mg
2 Options
Catalog number P2979
Price (USD)/ 1 mg
1,216.00
-
Add to cart
Quantity:
1 mg
Price (USD)/ 1 mg
1,216.00
Add to cart
Vivid™ DBOMF Substrate
Catalog numberP2979
Price (USD)/ 1 mg
1,216.00
-
Add to cart

Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product. They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. When used in combination with CYP450 BACULOSOMES™ Plus Reagents as part of the Vivid™ CYP450 Screening Kits, the Vivid™ Substrates enable rapid, robust detection of cytochrome P450 inhibitors. This particular substrate (Vivid ™ DBOMF Substrate) yields a product that emits a green fluorescence.

More About Vivid™ CYP450 Screening Kits

Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagent. CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Applications

  • High-throughput screening of enzyme-drug interactions
  • Compound profiling for drug inhibition of cytochrome P450 isozymes
  • Generation of predictive SAR models to guide compound acquisition
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Product LineVIVID™
Quantity1 mg
SubstrateDBOMF
Substrate PropertiesChemical Substrate
Substrate TypeCytochrome P450 Substrate
Shipping ConditionApproved for shipment on Wet or Dry Ice
Unit Size1 mg
Contents & Storage
Vivid™ CYP450 Screening kits include:
• Vivid™ CYP450 Fluorogenic substrates (also available separately)
• BACULOSOMES™ CYP450 reagents
• Fluorescent standards
• NADPH-regeneration system

Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)