A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Primary antibodies of the same isotype or derived from the same species can be multiplexed if they are directly labeled with compatible fluorophores. Using Pacific Blue and Pacific Orange dyes simultaneously allows you to excite both dyes at the same wavelength while their emissions are differentially distinguished.

Pacific Orange and Pacific Blue antibody labeling kits allow the labeling of small amounts of antibody with a violet light-excitable dye (up to 100 µg of antibody per reaction). Small amounts of other proteins (>40 kDa) can also be labeled using these kits.

Features of these antibody labeling kits
• Pacific Blue excitation and emission maxima: 410/455 nm
• Pacific Orange excitation and emission maxima: 405/551 nm
• Labeled proteins typically ready to use in 90 min. (∼15 min. hands-on time)
• Optimized for small-scale labeling of any protein >40 kDa
• Stabilizing proteins should be removed from the sample before labeling
• Purified using convenient spin filters
• Includes detailed instructions for determining degree of labeling (DOL)

These kits contain everything you need to perform five separate labeling reactions as well as to purify the resulting conjugates. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISA, immunocytochemistry, indirect FISH, and more. We are ISO 13485:2000 certified.