Pacific Orange™ Succinimidyl Ester, Triethylammonium Salt
Pacific Orange™ Succinimidyl Ester, Triethylammonium Salt
Invitrogen™

Pacific Orange™ Succinimidyl Ester, Triethylammonium Salt

Pacific Orange™ succinimidyl ester is a violet-excitable (405 nm) dye with emission maximum at ∼551 nm. This allows it toRead more
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Catalog NumberQuantity
P302531 mg
Catalog number P30253
Price (MXN)
-
Quantity:
1 mg

Pacific Orange™ succinimidyl ester is a violet-excitable (405 nm) dye with emission maximum at ∼551 nm. This allows it to be used with other violet-excitable fluorophores such as Pacific Blue™ dye for multiparameter analysis using a single excitation source. As a class of compounds, succinimidyl ester forms provide the most efficient and easy-to-use reaction chemistry to selectively link a dye to accessible primary amine groups on proteins, modified nucleic acids, or other biomolecules. Succinimidyl esters are excellent reagents for amine modification because the covalent bonds they form are as stable as the peptide bonds used to link amino acids in proteins. Pacific Orange™ succinimidyl ester is supplied as 1 mg of dry powder and should be stored at ≤-20°C, desiccated, and protected from light.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Emission551
Excitation405
Label or DyePacific Orange™
Product TypeSuccinimidyl Ester
Quantity1 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
Label TypePacific Dyes
Product LinePacific Orange
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Citations & References (7)

Citations & References
Abstract
Dual channel STED nanoscopy of lytic granules on actin filaments in natural killer cells.
Authors:Mace EM, Orange JS,
Journal:Commun Integr Biol
PubMed ID:22808328
Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized secretory lysosomes, termed lytic granules. This directed secretion is triggered following the formation of an immunological synapse (IS), which is characterized by actin re-modeling and receptor organization at the interface ... More
Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.
Authors:Clutter MR, Heffner GC, Krutzik PO, Sachen KL, Nolan GP,
Journal:Cytometry A
PubMed ID:20824632
'Intracellular flow cytometry permits quantitation of diverse molecular targets at the single-cell level. However, limitations in detection sensitivity inherently restrict the method, sometimes resulting in the inability to measure proteins of very low abundance or to differentiate cells expressing subtly different protein concentrations. To improve these measurements, an enzymatic amplification ... More
Dual receptor T cells mediate pathologic alloreactivity in patients with acute graft-versus-host disease.
Authors:Morris GP, Uy GL, Donermeyer D, Dipersio JF, Allen PM,
Journal:
PubMed ID:23740900
Acute graft-versus-host disease (aGVHD) results from a robust response of donor T cells transferred during hematopoietic stem cell transplantation (HSCT) to allogeneic peptide-major histocompatibility complex antigens. Previous investigations have not identified T cell subsets that selectively mediate either protective immunity or pathogenic alloreactivity. We demonstrate that the small subset of ... More
Activation of T lymphocytes in atherosclerotic plaques.
Authors:Grivel JC, Ivanova O, Pinegina N, Blank PS, Shpektor A, Margolis LB, Vasilieva E,
Journal:Arterioscler Thromb Vasc Biol
PubMed ID:21960562
To decipher the immunologic mechanisms of plaque maturation and rupture, it is necessary to analyze the phenotypes and distribution of individual lymphocytes that migrate to the plaques, as well as their activation at different stages of plaque formation. We developed a protocol to isolate plaque-residing immune cells and analyze their ... More
In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
Authors:McDonald JU, Cortini A, Rosas M, Fossati-Jimack L, Ling GS, Lewis KJ, Dewitt S, Liddiard K, Brown GD, Jones SA, Hallett MB, Botto M, Taylor PR,
Journal:FASEB J
PubMed ID:21368104
Mature neutrophils are notoriously short-lived immune cells that cannot be genetically manipulated. Analysis of gene function therefore requires genetically modified animals, which is expensive, time-consuming, and costly in animal life. Analysis of gene function in neutrophils in a physiologically relevant context thus represents a significant problem in the field. We ... More