Invitrogen™

Pro-Q™ Diamond Phosphoprotein Gel Stain

Catalog number:  P33300

 Related applications:

Protein Electrophoresis & Western Blotting

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Description

Pro-Q Diamond Phosphoprotein Gel Stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or use of phosphoprotein specific antibodies. It is ideal for the identification of kinase targets in signal transduction pathways and for phosphoproteomic studies. This fluorescent stain allows direct, in-gel detection of phosphate groups attached to tyrosine, serine, or threonine residues. Pro-Q Diamond Phosphoprotein Gel Stain can be used with standard SDS-polyacrylamide gels or with 2D gels.

The simple and reliable staining protocol delivers results in as little as 4 to 5 hours. The stain is also compatible with mass spectrometry, allowing analysis of the phosphorylation state of entire proteomes. Pro-Q Diamond Phosphoprotein Gel Stain can be used with SYPRO Ruby protein gel stain on the same gel for multiparameter staining.

Pro-Q Diamond Phosphoprotein Gel Stain detection is compatible with a visible light-scanning instruments, imaging equipment with appropriate filters, blue-LED transilluminators, or (with reduced sensitivity) 300-nm UV transilluminators.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Range or Limit: 1-16ng phosphoprotein/band
Target Molecule: Proteins (Phosphoproteins)
Label or Dye: Pro-Q® Diamond
Detection Method: Fluorescent
Detection Location: In-Gel Detection
Excitation⁄Emission (nm): 555⁄580
Product Line: PRO-Q®
Product Size: 1 L
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature and protect from light.

Documents

Manuals & protocols