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Citas y referencias (5)
Invitrogen™
Pro-Q™ Diamond Phosphoprotein Blot Stain Kit
El kit de tinción de transferencia de fosfoproteínas Pro-Q Diamond proporciona un método rápido y sencillo para la detección directaMás información
| Número de catálogo | Cantidad |
|---|---|
| P33356 | 1 Kit |
Número de catálogo P33356
Precio (MXN)
-
Cantidad:
1 Kit
El kit de tinción de transferencia de fosfoproteínas Pro-Q Diamond proporciona un método rápido y sencillo para la detección directa de fosfoproteínas en membranas de fluoruro de polivinilideno (PVDF) o de nitrocelulosa. La tinción fluorescente se une de forma selectiva a la fracción del fosfato y la unión es independiente del contexto de la secuencia del aminoácido. No se necesitan costosos anticuerpos o radioisótopos ya que se trata de una tinción directa. El kit de tinción de transferencia de fosfoproteínas Pro-Q Diamond se puede utilizar para analizar el estado de fosforilación nativo de las proteínas obtenidas de muestras de tejido o de fluidos corporales.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Ubicación de detecciónDetección en transferencia
Método de detecciónFluorescente
Línea de productosPRO-Q
Tipo de productoKit de tinción de transferencia de fosfoproteínas
Cantidad1 Kit
Duración de almacenamiento6 meses
Molécula dianaProteínas (fosfoproteínas)
Etiqueta o tintePro-Q Diamond
Unit SizeEach
Citations & References (5)
Citations & References
Abstract
The human mitochondrial transcription termination factor (mTERF) is fully active in vitro in the non-phosphorylated form.
Journal:J Biol Chem
PubMed ID:15899902
'The human mitochondrial transcription termination factor (mTERF) is a 39-kDa protein that terminates transcription at the 3''-end of the 16 S rRNA gene and thereby controls expression of the ribosomal transcription unit of mitochondrial DNA. The transcription termination activity of human mTERF has been notoriously difficult to study in vitro,
Ca(2+)-related signaling and protein phosphorylation abnormalities play central roles in a new experimental model of electrical storm.
Journal:Circulation
PubMed ID:21555709
Electrical storm (ES), characterized by recurrent ventricular tachycardia/fibrillation, typically occurs in implantable cardioverter-defibrillator patients and adversely affects prognosis. However, the underlying molecular basis is poorly understood. In the present study, we report a new experimental model featuring repetitive episodes of implantable cardioverter-defibrillator firing for recurrent ventricular fibrillation (VF), in which
Pin1 promotes histone H1 dephosphorylation and stabilizes its binding to chromatin.
Journal:
PubMed ID:24100296
Histone H1 plays a crucial role in stabilizing higher order chromatin structure. Transcriptional activation, DNA replication, and chromosome condensation all require changes in chromatin structure and are correlated with the phosphorylation of histone H1. In this study, we describe a novel interaction between Pin1, a phosphorylation-specific prolyl isomerase, and phosphorylated
Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using a novel fluorescent phosphorylation sensor dye.
Journal:Proteomics
PubMed ID:12872225
Ultrasensitive detection of minute amounts of phosphorylated proteins and peptides is a key requirement for unraveling many of the most important signal transduction pathways in mammalian systems. Protein microarrays are potentially useful tools for sensitive screening of global protein expression and post-translational modifications, such as phosphorylation. However, the analysis of
Detection of phosphoproteins on electroblot membranes using a small-molecule organic fluorophore.
Journal:Electrophoresis
PubMed ID:15300773
A new formulation of the small-molecule organic fluorophore, Pro-Q Diamond dye, has been developed that permits rapid and simple detection of phosphoproteins directly on polyvinylidene difluoride (PVDF) or nitrocellulose membranes (electroblots). Protein samples are first separated by electrophoresis and then electroblotted to membranes, stained and destained, in an analogous manner