The pHrodo Deep Red Antibody Labeling kit provides the materials needed to easily label and purify antibodies for the study of antibody internalization. Amine-reactive pHrodo Deep Red dye covalently binds to antibodies to produce bright, soluble conjugates that only fluoresce in acidic environments. The pHrodo Deep Red labeling kit makes it easier than ever to study antibody internalization with higher certainty of results and less optimization since pHrodo Deep Red dye only fluoresces in late endosomes and lysosomes.
pHrodo Deep Red Antibody Labeling Kit features include:
• Superior signal window
—minimal background at neutral pH enables maximal signal-to-noise ratio for certainty of results
—Cy5 channel allows for multicolor experiments
• Low pKa
—eliminates non-specific signal from non-internalized cargo and only fluoresces in late endosomes and lysosomes
—labeling typically takes 2 hours with less than 30 min hands-on time
pHrodo Deep Red is a low-background pH sensor dye that shows no signal in neutral conditions and only fluoresces in acidic environments. This unique property helps enable rapid assay development and helps increase certainty of results when investigating antibody internalization and endocytic/phagocytic pathways. pHrodo Deep Red dye helps enable better discrimination of internalized cargo from outside the cell because it has an approximate pKa of 5 and does not fluoresce until it enters the late endosome and lysosome. pHrodo Deep Red dye can be detected using a Cy5 fluorescent filter set and has been validated for use in a variety of applications, including flow cytometry, fluorescent microscopy, and high content analysis (HCA).
Learn more about pHrodo dyes
and antibody internalization
.Learn more about antibody labeling ›