Now also available in green color as pHrodo™ Green E. coli BioParticles® conjugate.
Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells

pHrodo® Red E. coli BioParticles® Conjugate for Phagocytosis

Catalog number: P35361

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry

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Description

New pH-sensitive pHrodo® dye conjugates give faster and more accurate results than any other phagocytosis assay.  pHrodo® dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes.  Get faster staining and more accurate results—without the need for wash steps or quencher dye.

• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments.
• Multiplex with green dyes such as GFP, Fluo-4, or calcein

The fluorescence of the novel pHrodo® dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-made pHrodo® E.coli BioParticles® conjugates in imaging or flow applications, or pHrodo® SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: E. coli
Label or Dye: pHrodo™ Red
Product Size: 5 x 2 mg
For Use With (Equipment): Fluorescence Microscope
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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Manuals & protocols

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