New pH-sensitive pHrodo™ dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo™ dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.
• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments.
• Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo™ dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-made pHrodo™ E.coli BioParticles™ conjugates in imaging or flow applications, or pHrodo™ SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.