Invitrogen™

pHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis

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Description

New pH-sensitive pHrodo® dye conjugates give faster and more accurate results than any other phagocytosis assay.  pHrodo® dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes.  Get faster staining and more accurate results—without the need for wash steps or quencher dye.

• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments.
• Multiplex with green dyes such as GFP, Fluo-4, or calcein

The fluorescence of the novel pHrodo® dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-made pHrodo® E.coli BioParticles® conjugates in imaging or flow applications, or pHrodo® SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type: Bioparticle Conjugate
For Use With (Equipment): Fluorescence Microscope
Label or Dye: pHrodo™ Red
Shipping Condition: Room Temperature
Product Line: BioParticles™, pHrodo™
Form: Solid
Species: E. coli

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.