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pHrodo® Green AM Intracellular pH Indicator

Catalog number: P35373

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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Description

pHrodo® Green AM Intracellular pH Indicator is a novel fluorogenic probe used to measure intracellular pH in live cells. pHrodo® Green is weakly fluorescent at neutral pH but increasingly fluorescent as the pH drops. This reagent can quantify cellular cytosolic pH in the range of 9-4 with a pKa of ~6.5 with excitation/emission of 509/533 nm. pHrodo® Green is photostable and appropriate for use in multiplexing experiments with red and deep red fluorophores such as RFP, Alexa Fluor® 594, MitoTracker® Red, and MitoTracker® Deep Red.

This pHrodo® Green dye has been modified with AM ester groups, which results in an uncharged molecule that can permeate cell membranes. Once inside the cell, the lipophilic blocking groups are cleaved by nonspecific esterases, resulting in a compound that is retained within the intracellular space. Fluorescence intensity of the probe is then an indicator of intracellular pH. Subsequent use of the Intracellular pH Calibration Buffer Kit (Cat. No. P35379) allows this intracellular pH to be quantified.

pHrodo® Green AM Intracellular pH Indicator is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometry, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos®, FLoid™, Tali™, and Attune™ instruments.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: pH Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Instrument
Cell Permeability: Cell-Permeant
Form: Liquid
Color: Green
Solubility: DMSO (Dimethylsulfoxide)
Product Size: 50 µL
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: Visible
Sub-Cellular Localization: Cytoplasm
Excitation⁄Emission (nm): 509⁄533

Contents & storage

Store at 2–8°C, dessicated and protected from light.

Documents

Manuals & protocols